AB181743

Anti-DIS3L2抗体[6C7B2]

Name: DIS3L2抗体[6C7B2] (ab181743)| Abcam中文官网
Brand: Abcam Limited
SKU: AB181743
Price: 4514 CNY
Availability: InStock

Anti-DIS3L2 antibody [6C7B2]

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(1 Publication)

Mouse Monoclonal DIS3L2 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DIS3L2 aa 1-250.

查看别名

FAM6A, DIS3L2, DIS3-like exonuclease 2, hDIS3L2

6 Images

Flow Cytometry - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Flow Cytometrical analysis of Jurkat cells labeling DIS3L2 with ab181743 at 1/200 (green) compared to a negative control antibody (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissue labeling DIS3L2 with ab181743 at 1/200.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemical analysis of paraffin embedded Human bladder cancer tissue labeling DIS3L2 with ab181743 at 1/200.

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

HepG2 cell lysate

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

All lanes:

DIS3L2 recombinant protein

Predicted band size: 27 kDa,68 kDa,99 kDa

false

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

DIS3L2 (aa 27-250)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 99 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

6C7B2

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-P, Flow Cyt, WB

applications

免疫原

Recombinant Fragment Protein within Human DIS3L2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q8IYB7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from tissue culture supernatant.

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DIS3L2 also known as DIS3-like exonuclease 2 is a protein involved in RNA processing and degradation. Mechanically it functions as a 3'-5' exonuclease meaning it removes nucleotides one by one from the 3' end of RNA molecules. The protein has a molecular mass of approximately 110 kDa. It is mainly expressed in the cytoplasm where it plays a critical role in maintaining RNA stability and turnover. DIS3L2 is present in various tissues with notable expression in the kidney and reproductive organs.

Biological function summary

The protein contributes to RNA metabolism by degrading small non-coding RNAs such as ribosomal RNA (rRNA) and transfer RNA (tRNA) fragments. It is not known to be part of a complex instead acting as an individual enzyme. DIS3L2 helps control the quantity and quality of RNA molecules in cells preventing accumulation of aberrant or excessive RNAs that can disrupt cellular processes.

Pathways

DIS3L2 participates in the RNA surveillance pathway ensuring proper RNA processing and maturation. This surveillance system involves several proteins that recognize and degrade abnormal RNAs therefore preserving genomic integrity. DIS3L2 collaborates with components of the exosome complex to achieve this function. In the regulatory RNAs pathway it interacts with proteins that manage microRNA decay thereby influencing gene expression by modulating microRNA levels.

Dysfunctional activity of DIS3L2 has been linked to Perlman syndrome a condition characterized by overgrowth and predisposition to Wilms tumor a type of kidney cancer. Studies suggest that DIS3L2 through its RNA degradation role impacts the balance of growth-regulatory factors. Additionally it has been associated with Bloom syndrome a disorder marked by genomic instability. Here the absence or malfunction of DIS3L2 can exacerbate the genomic instability that characterizes Bloom syndrome highlighting its interaction with pathways involving DNA repair proteins.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.

See full target information DIS3L2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:24728 PubMed27090737

2016

Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Yu Zhu,Wen-Dong Bai,Chao Li,Zhao Zheng,Hao Guan,Jia-Qi Liu,Xue-Kang Yang,Shi-Chao Han,Jian-Xin Gao,Hong-Tao Wang,Da-Hai Hu

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

Anti-DIS3L2抗体[6C7B2]

Anti-DIS3L2 antibody [6C7B2]

Flow Cytometry - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原