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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-dimethyl Arginine抗体[21C7] (ab413)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (11)

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Key features and details

  • Mouse monoclonal [21C7] to dimethyl Arginine
  • Suitable for: IP, WB, ChIP
  • Reacts with: Species independent
  • Isotype: IgM

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概述

  • 产品名称

    Anti-dimethyl Arginine抗体[21C7]
  • 描述

    小鼠单克隆抗体[21C7] to dimethyl Arginine
  • 宿主

    Mouse
  • 特异性

    This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
  • 经测试应用

    适用于: IP, WB, ChIPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

  • 常规说明

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.
  • 克隆

    单克隆
  • 克隆编号

    21C7
  • 同种型

    IgM
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgM H&L (ab9167)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab413于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent dilution.
WB (1)
1/50 - 1/500.
ChIP (1)
Use at an assay dependent concentration. Use 5µl for 4x106 cells.
说明
IP
Use at an assay dependent dilution.
WB
1/50 - 1/500.
ChIP
Use at an assay dependent concentration. Use 5µl for 4x106 cells.

靶标

  • 别名

    • Di methyl Arginine antibody

实验方案

  • ChIP protocols
  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab413?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab413 被引用在 11 文献中.

  • Franco CN  et al. Vitamin B6 is governed by the local compartmentalization of metabolic enzymes during growth. Sci Adv 9:eadi2232 (2023). PubMed: 37682999
  • Zhou CH  et al. Electroacupuncture Pretreatment Ameliorates PTSD-Like Behaviors in Rats by Enhancing Hippocampal Neurogenesis via the Keap1/Nrf2 Antioxidant Signaling Pathway. Front Cell Neurosci 13:275 (2019). PubMed: 31293390
  • Albrecht LV  et al. Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking. Proc Natl Acad Sci U S A 115:E5317-E5325 (2018). PubMed: 29773710
  • Yaseen I  et al. Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3. Nat Commun 6:8922 (2015). PubMed: 26568365
  • Slade DJ  et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). PubMed: 23576281
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

ChIP abreview for Anti-dimethyl Arginine antibody [21C7] - ChIP Grade

Excellent
Abreviews
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Balint Laszlo Balint

Verified customer

提交于 Oct 04 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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