AB124814

重组Anti-Dihydrofolate reductase (DHFR)抗体[EPR5285]

Name: Dihydrofolate reductase (DHFR)抗体[EPR5285] (ab124814)| Abcam中文官网
Brand: Abcam Limited
SKU: AB124814
Price: 2808 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

(1 Review)

(15 Publications)

Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

查看别名

Dihydrofolate reductase, DHFR

2 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue sections labeling Dihydrofolate reductase (DHFR) with purified ab124814 at 1/1000 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Unknown

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

All lanes:

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 4:

Y79 (Human retinoblastoma retinoblastoma) whole cell lysate

Lane 5:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 6:

Mouse spleen lysate

Lane 7:

Mouse kidney lysate

Lane 8:

Rat kidney lysate

Lane 9:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Secondary

Lanes 1 - 7:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 8 - 9:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 21 kDa

false

不同偶联物与剂型 (8)

Carrier free

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free
660 APC

APC Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
578 PE

PE Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5285

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner (PubMed : 12096917, PubMed : 15039552, PubMed : 17569517, PubMed : 19196009, PubMed : 19478082, PubMed : 21876184, PubMed : 9719595). Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway (PubMed : 21876188, PubMed : 22235121). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

See full target information Dihydrofolate reductase

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4342 PubMed40346061

2025

A general assay platform to study protein pharmacology using ligand-dependent structural dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel A Ciulla,Patricia K Dranchak,Mahesh Aitha,Renier H P van Neer,Divia Shah,Ravi Tharakan,Kelli M Wilson,Yuhong Wang,John C Braisted,James Inglese

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70391 PubMed39957677

2025

DHFR2 RNA directly regulates dihydrofolate reductase and its expression level impacts folate one carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Drago,Niamh Bookey,Kit-Yi Leung,Michael Henry,Paula Meleady,Nicholas D E Greene,Anne Parle-McDermott

International journal of molecular sciences 25: PubMed39337378

2024

Replicative Endothelial Cell Senescence May Lead to Endothelial Dysfunction by Increasing the BH2/BH4 Ratio Induced by Oxidative Stress, Reducing BH4 Availability, and Decreasing the Expression of eNOS.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio Hernandez-Navarro,Laura Botana,Javier Diez-Mata,Laura Tesoro,Beatriz Jimenez-Guirado,Claudia Gonzalez-Cucharero,Nunzio Alcharani,Jose Luis Zamorano,Marta Saura,Carlos Zaragoza

American journal of physiology. Gastrointestinal and liver physiology 327:G235-G253 PubMed38915277

2024

SGLT2 inhibition leads to a restoration of hepatic and circulating metabolites involved in the folate cycle and pyrimidine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ileana Mendez Espinoza,Elijah N D Choos,Carolyn M Ecelbarger,Blythe D Shepard

Cell chemical biology 31:221-233.e14 PubMed37875111

2023

Methotrexate-based PROTACs as DHFR-specific chemical probes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Rana,Patricia Dranchak,Jayme L Dahlin,Laurence Lamy,Wenqing Li,Erin Oliphant,Jonathan H Shrimp,Girish H Rajacharya,Ravi Tharakan,David O Holland,Apryl S Whitten,Kelli M Wilson,Pankaj K Singh,Scott K Durum,Dingyin Tao,Ganesha Rai,James Inglese

Frontiers in molecular neuroscience 16:1121944 PubMed37063365

2023

Increased NOX2 expression in astrocytes leads to eNOS uncoupling through dihydrofolate reductase in endothelial cells after subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hao Miao,Sheng-Qing Gao,Hui-Xin Li,Yun-Song Zhuang,Xue Wang,Tao Li,Chao-Chao Gao,Yan-Ling Han,Jia-Yin Qiu,Meng-Liang Zhou

Molecular cancer research : MCR 21:411-427 PubMed36669126

2023

BAP1 Loss Is Associated with Higher ASS1 Expression in Epithelioid Mesothelioma: Implications for Therapeutic Stratification.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Barnett,Jenna Kenyani,Martina Tripari,Zohra Butt,Rudi Grosman,Francesca Querques,Liam Shaw,Luisa C Silva,Zoe Goate,Stefan J Marciniak,Doris M Rassl,Richard Jackson,Lu-Yun Lian,Peter W Szlosarek,Joseph J Sacco,Judy M Coulson

Cellular and molecular life sciences : CMLS 79:553 PubMed36251090

2022

A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR.

Applications

Unspecified application

Species

Unspecified reactive species

Chiu-Yueh Hung,Chuanshu Zhu,Farooqahmed S Kittur,Maotao He,Erland Arning,Jianhui Zhang,Asia J Johnson,Gurpreet S Jawa,Michelle D Thomas,Tomas T Ding,Jiahua Xie

Frontiers in pharmacology 12:718675 PubMed34646134

2021

Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzo Sato,Masaru Tomita,Tomoyoshi Soga,Atsushi Ochiai,Hideki Makinoshima

Virus research 302:198469 PubMed34090962

2021

The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models.

Applications

Unspecified application

Species

Unspecified reactive species

Kim M Stegmann,Antje Dickmanns,Sabrina Gerber,Vella Nikolova,Luisa Klemke,Valentina Manzini,Denise Schlösser,Cathrin Bierwirth,Julia Freund,Maren Sitte,Raimond Lugert,Gabriela Salinas,Toni Luise Meister,Stephanie Pfaender,Dirk Görlich,Bernd Wollnik,Uwe Groß,Matthias Dobbelstein

View all publications

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重组Anti-Dihydrofolate reductase (DHFR)抗体[EPR5285]