重组Anti-Dihydrofolate reductase (DHFR)抗体[EPR5284] (ab133546)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Dihydrofolate reductase (DHFR)抗体[EPR5284]
参阅全部 Dihydrofolate reductase (DHFR) 一抗 -
描述
兔单克隆抗体[EPR5284] to Dihydrofolate reductase (DHFR) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. corresponding to Human Dihydrofolate reductase (DHFR) aa 1-100.
Database link: P00374 -
阳性对照
- HeLa, Jurkat, U937, 293T, C6, RAW 264.7 and PC-12 cell lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 1.28 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5284 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
靶标
-
功能
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. -
通路
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. -
序列相似性
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 1719 Human
- Entrez Gene: 13361 Mouse
- Entrez Gene: 24312 Rat
- Omim: 126060 Human
- SwissProt: P00374 Human
- SwissProt: P00375 Mouse
- SwissProt: Q920D2 Rat
- Unigene: 592364 Human
see all -
别名
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
see all
图片
-
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution (purified)
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab133546 被引用在 9 文献中.
- Lang MB et al. The actions of methotrexate on endothelial cells are dependent on the shear stress-induced regulation of one carbon metabolism. Front Immunol 14:1209490 (2023). PubMed: 37457690
- Lee SY et al. Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies. Biotechnol J 16:e2000351 (2021). PubMed: 33314785
- Tordonato C et al. miR-146 connects stem cell identity with metabolism and pharmacological resistance in breast cancer. J Cell Biol 220:N/A (2021). PubMed: 33819341
- Wu MT et al. MTHFR Knockdown Assists Cell Defense against Folate Depletion Induced Chromosome Segregation and Uracil Misincorporation in DNA. Int J Mol Sci 22:N/A (2021). PubMed: 34502300
- Gong L et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). PubMed: 30975976
- Ashkavand Z et al. Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype. Mol Cancer Res 15:189-200 (2017). WB ; Mouse . PubMed: 28108628
- Beane OS et al. Disparate Response to Methotrexate in Stem Versus Non-Stem Cells. Stem Cell Rev 12:340-51 (2016). PubMed: 26815725
- Thoms JA et al. Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr. Mol Cell Biol 36:1222-36 (2016). PubMed: 26830229
- Lee SW et al. Overexpression of thymidylate synthetase confers an independent prognostic indicator in nasopharyngeal carcinoma. Exp Mol Pathol 95:83-90 (2013). IHC ; Human . PubMed: 23726796