Anti-Digoxigenin抗体[21H8] (ab420)
Key features and details
- Mouse monoclonal [21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blot
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Digoxigenin抗体[21H8]
参阅全部 Digoxigenin 一抗 -
描述
小鼠单克隆抗体[21H8] to Digoxigenin -
宿主
Mouse -
特异性
Reacts with free and bound digoxigenin.
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经测试应用
适用于: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blotmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Digoxigenin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. -
存储溶液
Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
21H8 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000.
ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques |
|
| ELISA |
1/1000 - 1/10000.
|
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| IHC-P |
1/200 - 1/2000.
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| IHC-Fr |
1/200 - 1/2000.
|
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| In situ hybridization |
Use at an assay dependent concentration.
|
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| Southern Blot |
1/500 - 1/5000.
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| 说明 |
|---|
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WB
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques |
|
ELISA
1/1000 - 1/10000. |
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IHC-P
1/200 - 1/2000. |
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IHC-Fr
1/200 - 1/2000. |
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In situ hybridization
Use at an assay dependent concentration. |
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Southern Blot
1/500 - 1/5000. |
靶标
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相关性
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
别名
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab420 被引用在 40 文献中.
- Qian F et al. MiR-378a-3p as a putative biomarker for hepatocellular carcinoma diagnosis and prognosis: Computational screening with experimental validation. Clin Transl Med 11:e307 (2021). PubMed: 33634974
- Mann EK et al. Associations between DNA Damage and PD-L1 Expression in Ovarian Cancer, a Potential Biomarker for Clinical Response. Biology (Basel) 10:N/A (2021). PubMed: 33946684
- Samsonova A et al. Lin28, a major translation reprogramming factor, gains access to YB-1-packaged mRNA through its cold-shock domain. Commun Biol 4:359 (2021). PubMed: 33742080
- Shibata T et al. Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model. Nat Commun 12:236 (2021). PubMed: 33431896
- Cortes-Troncoso J et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight 5:N/A (2020). PubMed: 32376798