重组Anti-DHX8/prp22抗体[EPR9146(2)] (ab181074)
![Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)](https://www.abcam.cn/ps/products/181/ab181074/Images/ab181074-349234-anti-dhx8-prp22-antibody-epr9146-2-westernblot-human-mouse-rat.jpg "Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9146(2)] to DHX8/prp22
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-DHX8/prp22抗体[EPR9146(2)]
参阅全部 DHX8/prp22 一抗 -
描述
兔单克隆抗体[EPR9146(2)] to DHX8/prp22 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Y79, heLa, RAW 264.7, and PC-12 cell lysates and Jurkat whole cell lysate (ab7899);
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9146(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/20000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/20000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa). |
靶标
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功能
Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. -
序列相似性
Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 S1 motif domain. -
结构域
The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1659 Human
- Entrez Gene: 217207 Mouse
- Entrez Gene: 287727 Rat
- Omim: 600396 Human
- SwissProt: Q14562 Human
- SwissProt: A2A4P0 Mouse
- Unigene: 463105 Human
- Unigene: 28186 Mouse
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别名
- ATP dependent RNA helicase DHX8 antibody
- ATP-dependent RNA helicase dhx8 antibody
- DDX 8 antibody
see all
图片
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Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)All lanes : Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 139 kDa -
![Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)](https://www.abcam.cn/ps/products/181/ab181074/Images/ab181074-213112-WB.png)
Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/5000 dilution + Y79 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Exposure time: 30 seconds -
![Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)](https://www.abcam.cn/ps/products/181/ab181074/Images/ab181074-213110-anti-dhx8-antibody-epr91462-western-blot.jpg)
Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/20000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Exposure time: 3 minutes -
![Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)](https://www.abcam.cn/ps/products/181/ab181074/Images/ab181074-5-benefits-of-recombinant-antibodies.png)
Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab181074 被引用在 1 文献中.
- Chen X et al. High Levels of DEAH-Box Helicases Relate to Poor Prognosis and Reduction of DHX9 Improves Radiosensitivity of Hepatocellular Carcinoma. Front Oncol 12:900671 (2022). PubMed: 35814441