Anti-DHX36抗体(ab70269)
Key features and details
- Rabbit polyclonal to DHX36
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DHX36抗体
参阅全部 DHX36 一抗 -
描述
兔多克隆抗体to DHX36 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rhesus monkey, Gorilla, Orangutan
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免疫原
A region corresponding to C terminal amino acids 950-1008 of Human DHX36 (NP_065916.1)
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阳性对照
- WB: HeLa, HEK293T, Jurkat, mouse NIH3T3, TCKM, Renca whole cell lysates; IHC: human testicular seminoma, human testis;
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. -
组织特异性
Highly expressed in testis. -
序列相似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 170506 Human
- Entrez Gene: 72162 Mouse
- Omim: 612767 Human
- SwissProt: Q9H2U1 Human
- SwissProt: Q8VHK9 Mouse
- Unigene: 446270 Human
- Unigene: 224233 Mouse
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别名
- DDX36 antibody
- DEAH box protein 36 antibody
- Dhx36 antibody
see all
图片
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Western blot - Anti-DHX36 antibody (ab70269)All lanes : Anti-DHX36 antibody (ab70269) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : TCKM whole cell lysate
Lane 3 : Renca whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 115 kDa -
Western blot - Anti-DHX36 antibody (ab70269)All lanes : Anti-DHX36 antibody (ab70269) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HEK293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Predicted band size: 115 kDa -
Immunoprecipitation - Anti-DHX36 antibody (ab70269)Immunoprecipitation of HeLa whole cell lysate using ab70269
Lane 1: current lot ab70269
Lane 2: previous lot ab70269
Lane 3: Control IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX36 antibody (ab70269)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DHX36 with ab70269 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX36 antibody (ab70269)IHC image of ab70269 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70269, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab70269 被引用在 14 文献中.
- Chen X et al. Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. Cell Rep 39:110927 (2022). PubMed: 35675771
- Liu H et al. A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG Translation. J Am Chem Soc 143:7368-7379 (2021). PubMed: 33855846
- He L et al. DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease. J Cancer 11:893-905 (2020). PubMed: 31949493
- Herviou P et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nat Commun 11:2661 (2020). PubMed: 32461552
- Herdy B et al. Analysis of NRAS RNA G-quadruplex binding proteins reveals DDX3X as a novel interactor of cellular G-quadruplex containing transcripts. Nucleic Acids Res 46:11592-11604 (2018). PubMed: 30256975