Anti-DHX15/prp43抗体(ab70454)
Key features and details
- Rabbit polyclonal to DHX15/prp43
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DHX15/prp43抗体
参阅全部 DHX15/prp43 一抗 -
描述
兔多克隆抗体to DHX15/prp43 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human DHX15/prp43 (C terminal).
Database link: O43143 -
阳性对照
- 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70454 was affinity purified using an epitope specific to DHX15/prp43 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
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| IP |
Use at 1-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa). |
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IP
Use at 1-5 µg/mg of lysate. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 1665 Human
- Entrez Gene: 13204 Mouse
- Entrez Gene: 00174435 Orangutan
- Entrez Gene: 289693 Rat
- Omim: 603403 Human
- SwissProt: O43143 Human
- SwissProt: O35286 Mouse
- SwissProt: Q5RAZ4 Orangutan
see all -
别名
- ATP dependent RNA helicase #46 antibody
- ATP-dependent RNA helicase #46 antibody
- DBP1 antibody
see all
图片
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Western blot - Anti-DHX15/prp43 antibody (ab70454)All lanes : Anti-DHX15/prp43 antibody (ab70454) at 0.1 µg/ml
All lanes : Whole cell lysate from 293T cells.
Lysates/proteins at 60 µg per lane.
Predicted band size: 91 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-DHX15/prp43 antibody (ab70454)1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab70454 被引用在 8 文献中.
- Guo H et al. RNA helicase DHX15 exemplifies a unique dependency in acute leukemia. Haematologica 108:2029-2043 (2023). PubMed: 36861414
- Maul-Newby HM et al. A model for DHX15 mediated disassembly of A-complex spliceosomes. RNA 28:583-595 (2022). PubMed: 35046126
- Wang G et al. The RNA helicase DHX15 is a critical regulator of natural killer-cell homeostasis and functions. Cell Mol Immunol 19:687-701 (2022). PubMed: 35322175
- Zhao M et al. DHX15 Inhibits Autophagy and the Proliferation of Hepatoma Cells. Front Med (Lausanne) 7:591736 (2020). PubMed: 33644083
- Pattabhi S et al. DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection. J Interferon Cytokine Res 39:331-346 (2019). PubMed: 31090472
- Inesta-Vaquera F et al. DHX15 regulates CMTR1-dependent gene expression and cell proliferation. Life Sci Alliance 1:e201800092 (2018). PubMed: 30079402
- Zhu S et al. Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells. Nature 546:667-670 (2017). PubMed: 28636595
- Chen YL et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . PubMed: 24823796