重组Anti-DHODH抗体[EPR11814] (ab174288)
![Western blot - Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.cn/ps/products/174/ab174288/Images/ab174288-339731-ab174288WB1.jpg "Western blot - Anti-DHODH antibody [EPR11814] (ab174288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11814] to DHODH
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-DHODH抗体[EPR11814]
参阅全部 DHODH 一抗 -
描述
兔单克隆抗体[EPR11814] to DHODH -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human DHODH. The exact sequence is proprietary.
Database link: Q02127 -
阳性对照
- WB: SKBR-3 cell lysate. Human fetal heart tissue lysate. MCF7, HepG2, Human heart, Mouse heart, Rat heart and Jurkat whole cell lysate (ab7899).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11814 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 43 kDa.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 43 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. -
通路
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. -
疾病相关
Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. -
序列相似性
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. -
翻译后修饰
The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1723 Human
- Entrez Gene: 56749 Mouse
- Entrez Gene: 65156 Rat
- Omim: 126064 Human
- SwissProt: Q02127 Human
- SwissProt: O35435 Mouse
- SwissProt: Q63707 Rat
- Unigene: 654427 Human
see all -
别名
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
see all
图片
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Western blot - Anti-DHODH antibody [EPR11814] (ab174288)All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
![Western blot - Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.cn/ps/products/174/ab174288/Images/ab174288-199805-anti-dhodh-antibody-epr11814-western-blot.jpg)
Western blot - Anti-DHODH antibody [EPR11814] (ab174288)All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDaNote: ab174288 is diluted in 1% BSA.
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![Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.cn/ps/products/174/ab174288/Images/ab174288-3-benefits-of-recombinant-antibodies.png)
Anti-DHODH antibody [EPR11814] (ab174288)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab174288 被引用在 2 文献中.
- Wang X et al. Risk model-guided identification of MTDH expression as a marker for ferroptosis induction therapy in head and neck squamous cell carcinoma. Am J Cancer Res 13:5236-5253 (2023). PubMed: 38058815
- Hosseini M et al. UVB-induced DHODH upregulation, which is driven by STAT3, is a promising target for chemoprevention and combination therapy of photocarcinogenesis. Oncogenesis 8:52 (2019). PubMed: 31551419