Anti-DHHC-15抗体(ab121203)
Key features and details
- Rabbit polyclonal to DHHC-15
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DHHC-15抗体 -
描述
兔多克隆抗体to DHHC-15 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human DHHC-15 aa 238-322 (internal sequence).
Sequence:VSRNKTTLEAFCTPVFTSGPEKNGFNLGFIKNIQQVFGDKKKFWLIPIGS SPGDGHSFPMRSMNESQNPLLANEETWEDNEDDNQ
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阳性对照
- Human pancreas tissue; Human liver lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/100 - 1/250.
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| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/100 - 1/250. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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功能
Palmitoyltransferase specific for GAP43 and DLG4/PSD95. -
组织特异性
Expressed in placenta, liver, lung, kidney, heart and brain. -
疾病相关
Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
序列相似性
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
结构域
The DHHC domain is required for palmitoyltransferase activity. -
翻译后修饰
Autopalmitoylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 158866 Human
- Omim: 300576 Human
- SwissProt: Q96MV8 Human
- Unigene: 253211 Human
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别名
- DHHC-15 antibody
- Palmitoyltransferase ZDHHC15 antibody
- UNQ1969/PRO4501 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-DHHC-15 antibody (ab121203)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-15 antibody (ab121203)ab121203 at 1/5 dilution staining DHHC-15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
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Western blot - Anti-DHHC-15 antibody (ab121203)All lanes : Anti-DHHC-15 antibody (ab121203) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human Liver cell lysate
Lane 5 : Human tonsil cell lysate
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121203 被引用在 2 文献中.
- Abazari D et al. Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus. J Cell Sci 136:N/A (2023). PubMed: 37039765
- Shah BS et al. Regulation of dendrite morphology and excitatory synapse formation by zDHHC15. J Cell Sci 132:N/A (2019). PubMed: 31189538