Anti-DGKZ/DGK-zeta抗体(ab105195)
Key features and details
- Rabbit polyclonal to DGKZ/DGK-zeta
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DGKZ/DGK-zeta抗体
参阅全部 DGKZ/DGK-zeta 一抗 -
描述
兔多克隆抗体to DGKZ/DGK-zeta -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis, Medaka fish
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免疫原
Synthetic peptide corresponding to Human DGKZ/DGK-zeta aa 1067-1117.
Database link: NP_001099010.1 -
阳性对照
- HeLa and 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105195于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
靶标
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功能
Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. -
组织特异性
Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle. -
序列相似性
Belongs to the eukaryotic diacylglycerol kinase family.
Contains 2 ANK repeats.
Contains 1 DAGKc domain.
Contains 2 phorbol-ester/DAG-type zinc fingers. -
翻译后修饰
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 423197 Chicken
- Entrez Gene: 483635 Dog
- Entrez Gene: 100050499 Horse
- Entrez Gene: 8525 Human
- Entrez Gene: 104418 Mouse
- Entrez Gene: 81821 Rat
- Omim: 601441 Human
- SwissProt: Q13574 Human
see all -
别名
- DAG kinase zeta antibody
- DAGK5 antibody
- DAGK5 PEN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody (ab105195)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-DGKZ/DGK-zeta antibody (ab105195)All lanes : Anti-DGKZ/DGK-zeta antibody (ab105195) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab105195 被引用在 8 文献中.
- Takao S et al. Combined inhibition/silencing of diacylglycerol kinase α and ζ simultaneously and synergistically enhances interleukin-2 production in T cells and induces cell death of melanoma cells. J Cell Biochem 122:494-506 (2021). PubMed: 33399248
- González-Mancha N et al. Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse. Front Immunol 12:814570 (2021). PubMed: 35095913
- Arranz-Nicolás J et al. Diacylglycerol kinase ? limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes. J Immunother Cancer 8:N/A (2020). PubMed: 33246984
- Merino-Cortés SV et al. Diacylglycerol kinase ? promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse. Sci Signal 13:N/A (2020). PubMed: 32291315
- Stangl A et al. Regulation of the endosomal SNX27-retromer by OTULIN. Nat Commun 10:4320 (2019). PubMed: 31541095
- Jung IY et al. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res 78:4692-4703 (2018). PubMed: 29967261
- Torres-Ayuso P et al. Diacylglycerol kinase-? regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1. Oncogenesis 4:e164 (2015). PubMed: 26302180
- Gharbi SI et al. Transient PKCa shuttling to the immunological synapse is governed by DGK? and regulates L-selectin shedding. J Cell Sci 126:2176-86 (2013). PubMed: 23525016