AB248242

重组Anti-DGKE抗体[EPR7847] - BSA and Azide free

Anti-DGKE antibody [EPR7847] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal DGKE antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

DAGK5, DGKE, Diacylglycerol kinase epsilon, DAG kinase epsilon, Diglyceride kinase epsilon, DGK-epsilon

3 Images

Unknown

Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (AB248242)

This data was developed using ab128949, the same antibody clone in a different buffer formulation.

The cell lysates were prepared in 1%SDS Hot lysis method.

All lanes:

Western blot - Anti-DGKE antibody [EPR7847] (<a href='/products/primary-antibodies/dgke-antibody-epr7847-ab128949'>ab128949</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

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Lab

Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (AB248242)

This data was developed using ab128949, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

We recommend to use 1% SDS Hot lysis prepare method to get desired western blot results.

All lanes:

Western blot - Anti-DGKE antibody [EPR7847] (<a href='/products/primary-antibodies/dgke-antibody-epr7847-ab128949'>ab128949</a>) at 1/1000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

OI-RD Scanning - Anti-DGKE antibody [EPR7847] - BSA and Azide free (AB248242)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-DGKE antibody [EPR7847] - BSA and Azide free (AB248242)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Unconjugated

Anti-DGKE antibody [EPR7847]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7847

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab248242 is the carrier-free version of ab128949.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DGKE also known as diacylglycerol kinase epsilon is an enzyme that catalyzes the phosphorylation of diacylglycerol (DAG) to produce phosphatidic acid. DGKE has a molecular mass of approximately 63 kDa. It shows expression in multiple tissues with notable presence in kidney placenta and lung. In particular DGKE is found in endothelial cells and certain immune cells. DGKE functions as a significant regulator of lipid signaling influencing the balance of diacylglycerol and phosphatidic acid within cells.

Biological function summary

DGKE plays an important role in cellular signaling processes that involve DAG and phosphatidic acid. It is not known to be a component of a larger complex but acts independently to mediate its functions. By regulating DAG levels DGKE impacts several cellular processes such as membrane trafficking cytoskeletal reorganization and activation of protein kinase C. The enzyme also contributes to controlling cell growth and apoptosis by modulating downstream signaling pathways.

Pathways

DGKE is significantly involved in the phosphoinositide signaling pathway and the glycerophospholipid metabolism pathway. In these pathways DGKE interacts with other DAG kinases and phospholipase C. Through these interactions DGKE influences the generation of inositol trisphosphate and diacylglycerol which are important second messengers. Its role supports cellular responses to external stimuli thereby affecting cell proliferation differentiation and survival.

DGKE mutations have been linked to nephrotic syndrome particularly a type associated with early-onset disease called DGKE-associated nephrotic syndrome. Moreover DGKE is connected to thrombosis because its deficiency can lead to abnormal blood clotting. CD2AP another protein involved in the actin cytoskeleton and important for kidney function interacts with DGKE and is often studied together in the context of glomerular diseases. DGKE's relationship with these conditions highlights its importance in vascular and kidney health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 19744926, PubMed : 21477596, PubMed : 22108654, PubMed : 23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 8626589). Also plays an important role in the biosynthesis of complex lipids (PubMed : 8626589). Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed : 19744926, PubMed : 22108654, PubMed : 23274426). Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed : 22108654).

See full target information DGKE

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20220613 PubMed36694627

2023

circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Chunping Yang,Ping Li

View all publications

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重组Anti-DGKE抗体[EPR7847] - BSA and Azide free