重组Anti-DGAT1抗体[EPR13430] (ab178711)
![Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251040-anti-dgat1-antibody-epr13430-western-blot.jpg "Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13430] to DGAT1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DGAT1抗体[EPR13430]
参阅全部 DGAT1 一抗 -
描述
兔单克隆抗体[EPR13430] to DGAT1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and Jurkat whole cell lysates; Human fetal kidney lysate. ICC/IF: HEK-293 and HeLa cells. Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13430 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178711于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/250.
|
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| ICC/IF |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/250. |
|
ICC/IF
1/500. |
|
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
靶标
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功能
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
通路
Lipid metabolism; glycerolipid metabolism. -
序列相似性
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8694 Human
- Omim: 604900 Human
- SwissProt: O75907 Human
- Unigene: 521954 Human
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别名
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
图片
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Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251041-anti-dgat1-antibody-epr13430-western-blot.jpg)
Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/1000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251042-anti-dgat1-antibody-epr13430-western-blot.jpg)
Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711)Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/5000 dilution + Human fetal kidney tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251043-anti-dgat1-antibody-epr13430-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (ab178711)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling DGAT1 with ab178711 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab178711 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251044-anti-dgat1-antibody-epr13430-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (ab178711)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DGAT1 with ab178711 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab178711 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-251045-anti-dgat1-antibody-epr13430-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] (ab178711)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cellslabeling DGAT1 with ab178711 at 1/250 dilution (red) compared withwith a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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![Anti-DGAT1 antibody [EPR13430] (ab178711)](https://www.abcam.cn/ps/products/178/ab178711/Images/ab178711-7-benefits-of-recombinant-antibodies.png)
Anti-DGAT1 antibody [EPR13430] (ab178711)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
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