AB109445

重组Anti-Desmoplakin抗体[EPR4383(2)]

Name: Desmoplakin抗体[EPR4383(2)] (ab109445)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109445
Price: 2594 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-Desmoplakin antibody [EPR4383(2)]

RabMAb
Recombinant
了解详情

(2 Reviews)

(11 Publications)

Rabbit Recombinant Monoclonal DESP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.

查看别名

Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

3 Images

Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Unknown

Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

All lanes:

Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HACAT cell lysate at 10 µg

Lane 3:

BXPC-3 cell lysate at 10 µg

Predicted band size: 332 kDa

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不同偶联物与剂型 (8)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Desmoplakin antibody [EPR4383(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Desmoplakin antibody [EPR4383(2)]
Carrier free

Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free
578 PE

PE Anti-Desmoplakin antibody [EPR4383(2)]
660 APC

APC Anti-Desmoplakin antibody [EPR4383(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Desmoplakin antibody [EPR4383(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Desmoplakin antibody [EPR4383(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Desmoplakin antibody [EPR4383(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4383(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Desmoplakin is a protein that plays an integral role in linking intermediate filaments to desmosomal plaques. This protein is also known by its alternate name "DSP" and has a molecular mass of approximately 332 kDa. Desmoplakin is expressed in tissues where mechanical strength is necessary such as the heart and skin. Its expression ensures structural integrity and resilience in areas that experience substantial mechanical stress.

Biological function summary

Desmoplakin acts to maintain cell-cell adhesion through its involvement with desmosomes and is an essential component of the desmosomal complex. This complex is important for the architectural stability of epithelial and cardiac tissues. Desmoplakin interacts directly with other desmosomal proteins like plakoglobin and plakophilin forming a bridge between the cell membrane and the cytoskeleton.

Pathways

Desmoplakin is an important player in the maintenance and function of the cytoskeletal network particularly within the intermediate filament organization. In addition desmoplakin participates in cellular adhesion signaling pathways. Its interaction with proteins such as plakoglobin mediates the organization of desmosomes influencing cellular signaling and communication processes critical for tissue morphogenesis and repair.

Mutations or malfunctions of desmoplakin are linked to conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC) and skin disorders such as striate palmoplantar keratoderma. In these diseases desmoplakin's ability to maintain structural integrity is compromised leading to the disruption of tissue architecture. In ARVC for example the faulty interaction between desmoplakin and plakoglobin may cause abnormal heart muscle function contributing to the pathology of the disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity).

See full target information DSP

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 27: PubMed40879390

2025

Comprehensive analysis of desmosomal protein remodelling identifies desmocollin-2 as potential biomarker for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Zhenliang Hu,Lingmin Wu,Deniz Akdis,Weiquan Ye,Ardan M Saguner,Mingming Su,Hongbin Dong,Zhongli Chen,Dan Hu,Shengshou Hu,Firat Duru,Liang Chen

Science advances 8:eabo6566 PubMed35857525

2022

CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rhiannon B Werder,Tao Liu,Kristine M Abo,Jonathan Lindstrom-Vautrin,Carlos Villacorta-Martin,Jessie Huang,Anne Hinds,Nathan Boyer,Esther Bullitt,Marc Liesa,Edwin K Silverman,Darrell N Kotton,Michael H Cho,Xiaobo Zhou,Andrew A Wilson

Breast cancer research : BCR 24:7 PubMed35078507

2022

KRT13 promotes stemness and drives metastasis in breast cancer through a plakoglobin/c-Myc signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Yin,Qinlong Li,Stefan Mrdenovic,Gina Chia-Yi Chu,Boyang Jason Wu,Hong Bu,Peng Duan,Jayoung Kim,Sungyong You,Michael S Lewis,Gangning Liang,Ruoxiang Wang,Haiyen E Zhau,Leland W K Chung

International journal of molecular medicine 46:1765-1776 PubMed33000206

2020

Steroid receptor RNA activator inhibits the migration, invasion and stemness characteristics of renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chanjuan Zhang,Neng Zhu,Chan Liu,Hongtao Wu,Yantao Yin,Yaning Shi,Duanfang Liao,Li Qin

JCI insight 5: PubMed31194698

2019

Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald Ng,Heather Manring,Nikolaos Papoutsidakis,Taylor Albertelli,Nicole Tsai,Claudia J See,Xia Li,Jinkyu Park,Tyler L Stevens,Prameela J Bobbili,Muhammad Riaz,Yongming Ren,Christopher E Stoddard,Paul Ml Janssen,T Jared Bunch,Stephen P Hall,Ying-Chun Lo,Daniel L Jacoby,Yibing Qyang,Nathan Wright,Maegen A Ackermann,Stuart G Campbell

The Journal of investigative dermatology 139:1867-1875.e7 PubMed30905808

2019

Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Hiraku Suga,Tomonori Oka,Makoto Sugaya,Yasunari Sato,Tsuyoshi Ishii,Hiroyuki Nishida,Shumpei Ishikawa,Masashi Fukayama,Shinichi Sato

American journal of translational research 11:233-244 PubMed30787982

2019

Mechanical strain triggers differentiation of dental mesenchymal stem cells by activating osteogenesis-specific biomarkers expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ruofang Zhang,Jun Wan,Hongmei Wang

PloS one 12:e0185062 PubMed28934278

2017

Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Ortega,Estefanía Tarazón,Carolina Gil-Cayuela,María García-Manzanares,Luis Martínez-Dolz,Francisca Lago,José Ramón González-Juanatey,Juan Cinca,Esther Jorge,Manuel Portolés,Esther Roselló-Lletí,Miguel Rivera

The Journal of biological chemistry 289:17043-53 PubMed24782306

2014

Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes.

Applications

Species

Unspecified reactive species

Eva Hartlieb,Vera Rötzer,Mariya Radeva,Volker Spindler,Jens Waschke

PloS one 8:e53739 PubMed23326495

2013

Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion.

Applications

Species

Human

Eva Hartlieb,Bettina Kempf,Miriam Partilla,Balázs Vigh,Volker Spindler,Jens Waschke

View all publications

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重组Anti-Desmoplakin抗体[EPR4383(2)]