重组Anti-Desmoglein 3/PVA抗体[EPR7420] (ab128927)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7420] to Desmoglein 3/PVA
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Desmoglein 3/PVA抗体[EPR7420]
参阅全部 Desmoglein 3/PVA 一抗 -
描述
兔单克隆抗体[EPR7420] to Desmoglein 3/PVA -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Desmoglein 3/PVA aa 900-1000. The exact sequence is proprietary.
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阳性对照
- Human tonsil and A431 lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7420 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128927于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). |
靶标
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功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
Epidermis, tongue, tonsil, esophagus and carcinomas. -
序列相似性
Contains 4 cadherin domains. -
结构域
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 1830 Human
- Omim: 169615 Human
- SwissProt: P32926 Human
- Unigene: 1925 Human
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别名
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
see all
图片
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All lanes : Anti-Desmoglein 3/PVA antibody [EPR7420] (ab128927) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : A431 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab128927 被引用在 3 文献中.
- Wang X et al. Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis In Vitro and In Vivo. Front Immunol 13:886226 (2022). PubMed: 35911677
- Tie D et al. Case of bullous pemphigoid coexisting with anti-desmoglein autoantibodies. J Dermatol 44:822-825 (2017). PubMed: 28342216
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161