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重组RabMAb

重组Anti-Desmoglein 1/DSG1抗体[EPR6766(B)] (ab124798)

Reviews (5)Q&A (1)References (13)
Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
  • Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
  • Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6766(B)] to Desmoglein 1/DSG1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Desmoglein 1/DSG1抗体[EPR6766(B)]
    参阅全部 Desmoglein 1/DSG1 一抗

  • 描述

    兔单克隆抗体[EPR6766(B)] to Desmoglein 1/DSG1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Desmoglein 1/DSG1 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02413

  • 阳性对照

    • WB: Human skin lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124798于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

  • 组织特异性

    Epidermis, tongue, tonsil and esophagus.

  • 疾病相关

    Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.

  • 序列相似性

    Contains 4 cadherin domains.

  • 结构域

    Calcium may be bound by the cadherin-like repeats.

  • 细胞定位

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    图片

    • Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
      Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
      All lanes : Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/10000 dilution

      Lane 1 : Human skin lysate prepared in RIPA lysis method
      Lane 2 : Human skin lysate prepared in 1%SDS Hot lysis method

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

      Predicted band size: 114 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      ab181602 was used as GAPDH loading control.

      We recommend using 1%SDS Hot lysis prepare method to get desired Western Blot results.

    • Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
      Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
      Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 114 kDa

    • Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
      Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)

    文献 (13)

    发表研究结果有使用 ab124798？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124798 被引用在 13 文献中.

    • Cohen-Barak E  et al. Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion. Exp Dermatol 31:214-222 (2022). PubMed: 34379845
    • Uberoi A  et al. Commensal microbiota regulates skin barrier function and repair via signaling through the aryl hydrocarbon receptor. Cell Host Microbe 29:1235-1248.e8 (2021). PubMed: 34214492
    • Yi L  et al. Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC Pulm Med 21:252 (2021). PubMed: 34332565
    • Yoon JS  et al. Biophysical characteristics of dandruff-affected scalp categorized on the basis of sebum levels. J Cosmet Dermatol 20:1002-1008 (2021). PubMed: 32757243
    • Kashgari G  et al. Epithelial Migration and Non-adhesive Periderm Are Required for Digit Separation during Mammalian Development. Dev Cell 52:764-778.e4 (2020). PubMed: 32109382
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell (HMC)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    30 µg
    Specification
    HMC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 27 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 24 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (epidermis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Epidermis)
    Permeabilization
    No
    Specification
    Epidermis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Western blot abreview for Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (epidermis)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Question

    I spoke with antibody services this morning. he asked me to send protein sequences for desmoglein-1 and -3 so that we may find an antibody predictive of binding to these proteins in horse laminar tissue. Please call me or email me with any recommendations. Applications are : WB, IF (ICC and IHC on OCT sections), Flow Cytometry.

    Read More
    Answer

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.

    The catalogue number is ab124798:

    Click here (or use the following: https://www.abcam.com/Desmoglein-1DSG1-antibody-EPR6766B-ab124798.html).

    The immunogen sequence is 93% conserved in the horse sequence you sent (14 of 15 amino acids are identical), so it is likely that ab124798 will cross-react with the DSG1-like horse protein. The antibody has not been tested in IHC on frozen tissue sections, only paraffin-embedded tissue. It has not been tested by flow cytometry, but does work in immunocytochemistry, and is likely to work for flow cytometry as well.

    For DSG3, the best candidate is ab14416, a mouse monoclonal raised against the extracellular portion of human DSG3. This region is 84% conserved in the horse sequence you sent. We do not know where the epitope that ab14416 recognizes falls within the extracellular region, so it is impossible to say if the epitope (generally consisting of a 6 - 8 amino acid sequence) is more or less than 84% identical to the homologous horse sequence. Again, the antibody has not been tested in all the applications you specified.

    Click here (or use the following: https://www.abcam.com/Desmoglein-3PVA-antibody-5G11-ab14416.html).

    I hope this helps. Please let me know if you have any questions.

    Read More

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