Anti-Desmoglein 1/DSG1抗体[32-2B] (ab108490)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [32-2B] (ab108490)](https://www.abcam.cn/ps/products/108/ab108490/Images/ab108490-196805-ab108490gr797451edited.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [32-2B] (ab108490)")
Key features and details
- Mouse monoclonal [32-2B] to Desmoglein 1/DSG1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Desmoglein 1/DSG1抗体[32-2B]
参阅全部 Desmoglein 1/DSG1 一抗 -
描述
小鼠单克隆抗体[32-2B] to Desmoglein 1/DSG1 -
宿主
Mouse -
特异性
This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to Desmoglein 1/DSG1.
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
32-2B -
骨髓瘤
NS1 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108490于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
Epidermis, tongue, tonsil and esophagus. -
疾病相关
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
序列相似性
Contains 4 cadherin domains. -
结构域
Calcium may be bound by the cadherin-like repeats. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 1828 Human
- Omim: 125670 Human
- SwissProt: Q02413 Human
- Unigene: 2633 Human
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别名
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [32-2B] (ab108490)
IHC image of Desmoglein 1/DSG1 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108490 被引用在 2 文献中.
- Conn IG et al. Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas. Br J Urol 65:176-80 (1990). PubMed: 2317652
- Vilela MJ et al. Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology. J Pathol 153:365-75 (1987). PubMed: 3323436