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重组RabMAb

重组Anti-Desmocollin 3抗体[EPR7486] (ab126768)

Submit a review Submit a question References (3)
Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
  • Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
  • Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
  • OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
  • Anti-Desmocollin 3 antibody [EPR7486] (ab126768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7486] to Desmocollin 3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Desmocollin 3抗体[EPR7486]
    参阅全部 Desmocollin 3 一抗

  • 描述

    兔单克隆抗体[EPR7486] to Desmocollin 3

  • 宿主

    Rabbit

  • 特异性

    This antibody cross-reacts weakly with Desmocollin 2.

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • A431, Human skin, and Human espophagus lysates; and A431 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 解离常数（KD）

    KD = 2.40 x 10 -11 M
    Learn more about KD

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR7486

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126768于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
应用说明
Is unsuitable for IHC-P or IP.

靶标

  • 功能

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.

  • 组织特异性

    Epidermis, buccal mucosa, esophagus and cervix.

  • 疾病相关

    Defects in DSC3 are the cause of hypotrichosis and recurrent skin vesicles (HRSV) [MIM:613102]. A disorder characterized by hypotrichosis and the appearance of recurrent skin vesicle formation. Affected individuals show sparse and fragile hair on scalp, as well as absent eyebrows and eyelashes. Vesicles filled with thin, watery fluid are observed on the scalp and skin of most of the boby. Mucosal vesicles are absent.

  • 序列相似性

    Contains 5 cadherin domains.

  • 结构域

    Calcium may be bound by the cadherin-like repeats.

  • 细胞定位

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cadherin family member 3 antibody
      • CDHF3 antibody
      • desmocollin 3a/3b antibody
      • Desmocollin 4 antibody
      • Desmocollin-3 antibody
      • Desmocollin-4 antibody
      • Desmocollin3 antibody
      • Desmocollin4 antibody
      • DSC antibody
      • DSC1 antibody
      • DSC2 antibody
      • DSC3 antibody
      • DSC3_HUMAN antibody
      • DSC4 antibody
      • HT CP antibody
      • HT-CP antibody
      • HTCP antibody
      see all

    图片

    • Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/200000 dilution

      Lane 1 : Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate
      Lane 3 : 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate
      Lane 4 : 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa


      Exposure time: 40 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)

      ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

       

    • Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Human skin lysate
      Lane 3 : Human espophagus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 100 kDa

    • OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
      Anti-Desmocollin 3 antibody [EPR7486] (ab126768)

    文献 (3)

    发表研究结果有使用 ab126768？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab126768 被引用在 3 文献中.

    • Wang J  et al. The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma. Pulm Pharmacol Ther 63:101931 (2020). PubMed: 32818548
    • Bir F  et al. New immunohistochemical markers in the differential diagnosisof nonsmall cell lung carcinoma. Turk J Med Sci 46:1854-1861 (2016). PubMed: 28081338
    • Passantino L  et al. Sodium metavanadate exhibits carcinogenic tendencies in vitro in immortalized human bronchial epithelial cells. Metallomics 5:1357-67 (2013). WB ; Human . PubMed: 23963610

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