Anti-Desmin抗体[DE-U-10] - Cytoskeleton Marker (ab6322)
Key features and details
- Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
-
产品名称
Anti-Desmin抗体[DE-U-10] - Cytoskeleton Marker
参阅全部 Desmin 一抗 -
描述
小鼠单克隆抗体[DE-U-10] to Desmin - Cytoskeleton Marker -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake -
免疫原
Full length native protein (purified) corresponding to Pig Desmin.
-
阳性对照
- IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
DE-U-10 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
疾病相关
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 280765 Cow
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P02542 Chicken
- SwissProt: O62654 Cow
- SwissProt: P17661 Human
see all -
别名
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on mouse cardiac muscle.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on smooth muscle of rat colon.
-
IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
数据表及文件
-
SDS download
-
Datasheet download
文献 (55)
ab6322 被引用在 55 文献中.
- Groppa E et al. Spatial compartmentalization of signaling imparts source-specific functions on secreted factors. Cell Rep 42:112051 (2023). PubMed: 36729831
- Jiang K et al. Diverse myopathological features in the congenital myasthenia syndrome with GFPT1 mutation. Brain Behav 12:e2469 (2022). PubMed: 34978387
- Claeyssen C et al. Global O-GlcNAcylation changes impact desmin phosphorylation and its partition toward cytoskeleton in C2C12 skeletal muscle cells differentiated into myotubes. Sci Rep 12:9831 (2022). PubMed: 35701470
- Miyata R et al. Lung fibrogenic microenvironment in mouse reconstitutes human alveolar structure and lung tumor. iScience 25:104912 (2022). PubMed: 36060050
- Goto N et al. Lymphatics and fibroblasts support intestinal stem cells in homeostasis and injury. Cell Stem Cell 29:1246-1261.e6 (2022). PubMed: 35931033