Anti-DERL1/Derlin-1抗体(ab176732)
Key features and details
- Rabbit polyclonal to DERL1/Derlin-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DERL1/Derlin-1抗体
参阅全部 DERL1/Derlin-1 一抗 -
描述
兔多克隆抗体to DERL1/Derlin-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Non human primates
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免疫原
Synthetic peptide within Human DERL1/Derlin-1 aa 201-251. The exact sequence is proprietary. NP_077271.1.
Database link: Q9BUN8 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 cell lysates. IP: HeLa cell lysate. IHC-P: Human ovarian carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176732 is affinity purified using an epitope specific to DERL1/Derlin-1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 29 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 29 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Derlin 1 is a channel-like protein found in the endoplasmic reticulum (ER) membrane. It is specifically required for the degradation of misfolded ER luminal proteins. Derlin 1 participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. It may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol and then transferring them to the ATPase VCP complex, which translocates and ubiquitinates misfolded proteins. In the case of infection by cytomegaloviruses, derlin 1 plays a central role in export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Derlin 1 also participates in the degradation of misfolded cytomegalovirus US2 protein. -
细胞定位
Endoplasmic reticulum; multi pass membrane protein. -
数据库链接
- Entrez Gene: 420350 Chicken
- Entrez Gene: 404121 Cow
- Entrez Gene: 475086 Dog
- Entrez Gene: 100727484 Guinea pig
- Entrez Gene: 79139 Human
- Entrez Gene: 67819 Mouse
- Entrez Gene: 100626802 Pig
- Entrez Gene: 362912 Rat
see all -
别名
- DER 1 antibody
- DER1 antibody
- Der1 like protein 1 antibody
see all
图片
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Western blot - Anti-DERL1/Derlin-1 antibody (ab176732)All lanes : Anti-DERL1/Derlin-1 antibody (ab176732) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-DERL1/Derlin-1 antibody (ab176732)ab176732 at 0.4 µg/ml detecting DERL1/Derlin-1 in HeLa whole cell lysate by WB following IP. ab176732 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DERL1/Derlin-1 antibody (ab176732)Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling DERL1/Derlin-1 using ab176732 at a 1/200 dilution, followed by peroxidase substrate detection.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab176732 被引用在 8 文献中.
- Zhang TM et al. TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression. Autophagy 19:805-821 (2023). PubMed: 35920704
- Li S et al. Abnormally decreased renal Klotho is linked to endoplasmic reticulum-associated degradation in mice. Int J Med Sci 19:321-330 (2022). PubMed: 35165517
- McDonald EF et al. Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants. Mol Biol Cell 33:ar62 (2022). PubMed: 35389766
- McKenna MJ et al. ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins. Mol Cell 82:4277-4289.e10 (2022). PubMed: 36283413
- Rao B et al. The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1. Sci Adv 7:N/A (2021). PubMed: 33658201
- Yang F et al. Preservation of endoplasmic reticulum (ER) Ca2+ stores by deletion of inositol-1,4,5-trisphosphate receptor type 1 promotes ER retrotranslocation, proteostasis, and protein outer segment localization in cyclic nucleotide-gated channel-deficient cone photoreceptors. FASEB J 35:e21579 (2021). PubMed: 33960001
- Yang F et al. Potential contribution of ryanodine receptor 2 upregulation to cGMP/PKG signaling-induced cone degeneration in cyclic nucleotide-gated channel deficiency. FASEB J 34:6335-6350 (2020). PubMed: 32173907
- Zeng J et al. Derlin-1 exhibits oncogenic activities and indicates an unfavorable prognosis in breast cancer. Cell Biol Int 44:593-602 (2020). PubMed: 31670413
