Anti-DENND2D抗体(ab184799)
Key features and details
- Rabbit polyclonal to DENND2D
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DENND2D抗体
参阅全部 DENND2D 一抗 -
描述
兔多克隆抗体to DENND2D -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human DENND2D aa 350 to the C-terminus.
Database link: Q9H6A0 -
阳性对照
- ICC: A-431 whole cells; IHC-P: Human stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184799于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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说明 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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序列相似性
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain. - Information by UniProt
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数据库链接
- Entrez Gene: 79961 Human
- Entrez Gene: 72121 Mouse
- Entrez Gene: 310772 Rat
- Omim: 615111 Human
- SwissProt: Q9H6A0 Human
- SwissProt: Q91VV4 Mouse
- Unigene: 557850 Human
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别名
- 2010308M01Rik antibody
- AI451421 antibody
- DEN2D_HUMAN antibody
see all
图片
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Immunofluorescence analysis of A431 (human epidermoid carcinoma cell line) whole cells labeling DENND2D in the cytosol with ab184799 at 2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling DENND2D in the cytoplasm of glandular cells with ab184799 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab184799 被引用在 1 文献中.
- Ma WJ et al. Stage IV colon cancer patients without DENND2D expression benefit more from neoadjuvant chemotherapy. Cell Death Dis 13:439 (2022). PubMed: 35523764