Anti-Dengue Virus E glycoprotein抗体[DE1] (ab41349)

Thank you for your enquiry. Basically, the epitopes for DE1 and DE2 are complex, conformational epitopes and probably non-linear - and since the immunogen is not a peptide, the amino acid sequence is not known. The information on the recombinant DIII coated plate is just additional information. Both monoclonal antibodies work with native antigens and may work with recombinant proteins which are folded properly. It may be that our recombinant DIII has not folded perfectly and therefore, both monoclonal antibodies did not work with our recombinant DIII. Unfortunately, there is no further information on this failed testing. I am sorry I can't provide more information as there is no more info on the Domain III testing results. If you have any other questions pertaining to ab41349 or ab41696, then please do not hesitate to contact me. I will do my best to locate more information for you. Have a nice day.

Unfortunately, we do not know where the epitope for DE1 is on the E glycoprotein. However, we do know that it does not bind recombinant DIII from the pET expression system. This therefore suggests that this antibody will not recognise endogenous domain III.

Thank you for contacting us.

We searched on our web page and there are three products that I could recommend to you. They are all three against the IgG2b of mouse and were used for ICC/IF. The species the three products were raised in is different and that might be important for you and will need some consideration. For full information about our products please see the three data sheets below:


https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-ab97249.html


https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-pre-adsorbed-ab98702.html

https://www.abcam.com/Rat-monoclonal-LO-MG2b-2-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-ab99637.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

ab41349 is a tissue culture supernatant therefore it is normal for it to be reddish as this may be due to the colour of the media. It is likely that there are additional reagents and/or proteins that might obliterate the absorption at 280 nm.

Since the antibody has not been purified, the concentration has not been determined. However, we predict that the concentration will be 1-3 ml/ml.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your response.

Currently, we have antibodies against Dengue virus which detect Type 1 and Type 3 separately.

If you need any further assistance, please do not hesitate to contact me.

Thank you for your enquiry and your interest in our products.

I can confirm that the Western blot results with this antibody (ab41349: Anti-Dengue Virus E glycoprotein antibody [DE1]) have been inconclusive, thus we do not list WB as a tested application on the on-line product datasheet.

I have conducted a search for you and found another antibody which may be suitable for you. I would advise you please to read the datasheet carefully to see if this would suit your need:

ab80914: https://www.abcam.com/dengue-virus-e-glycoprotein-antibody-8-ab80914.html

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your enquiry. DE1 (ab41349) and DE2 (ab41696) are monoclonal antibodies from two different fusion experiments but the immunizing protocol is the same for both fusions. As mentioned in the datasheets, although they are both dengue group specific, DE1 is the monoclonal that is currently being used in the MAC-ELISA for detection of dengue-specific IgM. We believe that DE2 will function just as well in the MAC-ELISA but extensive comparisons have not been carried out. Additionally, we have tested them on recombinant DIII coated plates and they were not reactive. I hope this information will be useful. If there is anything else that I can help you with, please do not hesitate to contact me.