Anti-DeltaD抗体[Dld 5C7/2，zdd2] (ab73331)
Key features and details
- Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cyt
- Reacts with: Zebrafish
- Isotype: IgG1
概述
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产品名称
Anti-DeltaD抗体[Dld 5C7/2，zdd2] -
描述
小鼠单克隆抗体[Dld 5C7/2，zdd2] to DeltaD -
宿主
Mouse -
特异性
This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA. -
经测试应用
适用于: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cytmore details -
种属反应性
与反应: Zebrafish -
免疫原
Extracellular DeltaD peptide (Zebrafish).
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表位
Zebrafish DeltaD protein extracellular domain. -
阳性对照
- HEK293 cells transfected with deltaD or zebrafish embryo.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Dld 5C7/2, zdd2 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC - Wholemount | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC - Wholemount
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
DeltaD is one of the 4 members of the zebrafish Delta family of Notch ligands, important in the control of cell differentiation in many tissues. DeltaD acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. It can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized. -
细胞定位
Cell Membrane -
数据库链接
- Entrez Gene: 30138 Zebrafish
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别名
- Aei antibody
- After eight antibody
- Dld antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab73331 被引用在 12 文献中.
- Zhao X et al. Colocalization Analysis for Cryosectioned and Immunostained Tissue Samples with or without Label Retention Expansion Microscopy (LR-ExM) by JACoP. Bio Protoc 12:e4336 (2022). PubMed: 35592606
- Zhao X et al. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Sci Adv 7:N/A (2021). WB, ICC ; Zebrafish . PubMed: 34117063
- Sehgal P et al. LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy. EMBO J 40:e107134 (2021). PubMed: 34180064
- Sharma P et al. Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis. Sci Rep 9:9958 (2019). PubMed: 31292468
- Gallagher TL et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). PubMed: 28648842