Anti-delta Sarcoglycan抗体(ab115019)
Key features and details
- Goat polyclonal to delta Sarcoglycan
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-delta Sarcoglycan抗体
参阅全部 delta Sarcoglycan 一抗 -
描述
山羊多克隆抗体to delta Sarcoglycan -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow, Dog, Pig
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免疫原
Synthetic peptide:
C-RLPHGSYTPTGTR
, corresponding to internal sequence amino acids 245-257 of Human delta Sarcoglycan(NP_000328.2; NP_758447.1; NP_001121681.1). -
阳性对照
- Human and Rat heart lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab115019 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115019于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. -
组织特异性
Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung. -
疾病相关
Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Belongs to the sarcoglycan beta/delta/gamma/zeta family. -
翻译后修饰
Glycosylated.
Disulfide bonds are present. -
细胞定位
Cell membrane > sarcolemma. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 6444 Human
- Entrez Gene: 24052 Mouse
- Omim: 601411 Human
- SwissProt: Q92629 Human
- SwissProt: P82347 Mouse
- Unigene: 387207 Human
- Unigene: 644733 Human
- Unigene: 338890 Mouse
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别名
- 35 kDa dystrophin associated glycoprotein antibody
- 35 kDa dystrophin-associated glycoprotein antibody
- 35DAG antibody
see all
图片
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Western blot - Anti-delta Sarcoglycan antibody (ab115019)Anti-delta Sarcoglycan antibody (ab115019) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115019 尚未被引用在任何文献中。