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重组RabMAb

重组Anti-Delta Opioid Receptor抗体[EPR5029(2)] (ab176324)

Submit a review Submit a question References (12)
Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
  • Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Delta Opioid Receptor抗体[EPR5029(2)]
    参阅全部 Delta Opioid Receptor 一抗

  • 描述

    兔单克隆抗体[EPR5029(2)] to Delta Opioid Receptor

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt (Intra), ICC/IFmore details
    不适用于: IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P41143

  • 阳性对照

    • WB: Human brain, Mouse spleen, Rat spleen, and Mouse brain lysates ICC/IF: SH-SY5Y cells. Flow Cyt: U-87 MG cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR5029(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176324于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt (Intra)
1/10 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/500.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt (Intra)
1/10 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/500.
应用说明
Is unsuitable for IHC-P or IP.

靶标

  • 相关性

    Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.

  • 细胞定位

    Multi pass membrane protein

  • 数据库链接

    see all

  • 别名

    • D-OR-1 antibody
    • Delta type opioid receptor antibody
    • Delta type opioid receptor DOR 1 antibody
    • Delta type opioid receptor DOR1 antibody
    • Delta-type opioid receptor antibody
    • DOR 1 antibody
    • DOR-1 antibody
    • DOR1 antibody
    • mDOR antibody
    • Nbor antibody
    • Opioid receptor delta 1 antibody
    • OPRD 1 antibody
    • OPRD antibody
    • OPRD_HUMAN antibody
    • Oprd1 antibody
    see all

图片

  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/2000 dilution (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/10000 dilution (Purified) + Mouse brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : U87-MG lysate
    Lane 4 : HUVEC lysate
    Lane 5 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

    Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

     

    Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor®488 Goat anti-Rabbit secondary IgG (1/1000).

  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

    Flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red) or a rabbit IgG control (green).

  • Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

文献 (12)

发表研究结果有使用 ab176324？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab176324 被引用在 12 文献中.

  • Zhang J  et al. Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice. iScience 26:107536 (2023). PubMed: 37636073
  • Manai F  et al. Gluten Exorphins Promote Cell Proliferation through the Activation of Mitogenic and Pro-Survival Pathways. Int J Mol Sci 24:N/A (2023). PubMed: 36835317
  • Zhu Y  et al. Nucleus accumbens D1/D2 circuits control opioid withdrawal symptoms in mice. J Clin Invest 133:N/A (2023). PubMed: 37561576
  • Xu Y  et al. A Critical Role of δ-Opioid Receptor in Anti-microglial Activation Under Stress. Front Aging Neurosci 14:847386 (2022). PubMed: 35663569
  • Zhang R  et al. Delta Opioid Peptide Targets Brain Microvascular Endothelial Cells Reducing Apoptosis to Relieve Hypoxia-Ischemic/Reperfusion Injury. Pharmaceutics 15:N/A (2022). PubMed: 36678674
View all Publications for this product

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