Anti-delta 1 Catenin/CAS (phospho Y291)抗体[EP2385Y] (ab81274)
![Western blot - Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)](https://www.abcam.cn/ps/products/81/ab81274/Images/ab81274-96456-anti-delta-1-catenin-cas-phospho-y291-antibody-ep2385y-western-blot.jpg "Western blot - Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)")
Key features and details
- Rabbit monoclonal [EP2385Y] to delta 1 Catenin/CAS (phospho Y291)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-delta 1 Catenin/CAS (phospho Y291)抗体[EP2385Y]
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔单克隆抗体[EP2385Y] to delta 1 Catenin/CAS (phospho Y291) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat cell lysate treated with pervanadate
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2385Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81274于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/20000. Predicted molecular weight: 108 kDa.
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| 说明 |
|---|
|
WB
1/10000 - 1/20000. Predicted molecular weight: 108 kDa. |
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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Western blot - Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)All lanes : Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274) at 1/20000 dilution
Lane 1 : Jurkat cell lysate untreated
Lane 2 : Jurkat cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab81274 尚未被引用在任何文献中。