重组Anti-delta 1 Catenin/CAS (phospho Y280)抗体[EPR2384(2)] (ab173578)
![Western blot - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)](https://www.abcam.cn/ps/products/173/ab173578/Images/ab173578-197108-anti-delta-1-catenin-cas-phospho-y280-antibody-epr23842-western-blot.jpg "Western blot - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2384(2)] to delta 1 Catenin/CAS (phospho Y280)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-delta 1 Catenin/CAS (phospho Y280)抗体[EPR2384(2)]
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔单克隆抗体[EPR2384(2)] to delta 1 Catenin/CAS (phospho Y280) -
宿主
Rabbit -
特异性
This antibody only detects p120 Catenin when phosphorylated at Tyrosine 280
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经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human delta 1 Catenin/CAS aa 250-350 (phospho Y280) (Cysteine residue). The exact sequence is proprietary.
Database link: O60716 -
阳性对照
- Pervanadate treated A431 cell lysate, Human gastric adenocarcinoma
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Do Not Freeze. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2384(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000 - 1/20000. Predicted molecular weight: 90-120 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| 说明 |
|---|
|
WB
1/50000 - 1/20000. Predicted molecular weight: 90-120 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Entrez Gene: 311163 Rat
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
- Unigene: 35738 Mouse
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别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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Western blot - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)All lanes : Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578) at 1/50000 dilution
Lane 1 : Untreated A431 cell lysate
Lane 2 : Pervanadate treated A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 90-120 kDa -
![Immunoprecipitation - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)](https://www.abcam.cn/ps/products/173/ab173578/Images/ab173578-197110-anti-delta-1-catenin-cas-phospho-y280-antibody-epr23842-immunoprecipitation.jpg)
Immunoprecipitation - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)Western blot analysis on immunoprecipitation pellet from A431 cells treated with pervanadate lysate: ab173578 at 1/10 dilution immunoprecipitated delta 1 Catenin/CAS (phospho Y280).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)](https://www.abcam.cn/ps/products/173/ab173578/Images/ab173578-197109-anti-delta-1-catenin-cas-phospho-y280-antibody-epr23842-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling delta 1 Catenin/CAS (phospho Y280) with ab173578 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)](https://www.abcam.cn/ps/products/173/ab173578/Images/ab173578-4-benefits-of-recombinant-antibodies.png)
Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab173578 被引用在 1 文献中.
- Ma L et al. Overexpression of PTPRZ1 Regulates p120/β-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis. J Oncol 2022:2352360 (2022). PubMed: 35251170