重组Anti-delta 1 Catenin/CAS (phospho S268)抗体[EPR2380] (ab79545)
![Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.cn/ps/products/79/ab79545/Images/ab79545-95095-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-western-blot.jpg "Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2380] to delta 1 Catenin/CAS (phospho S268)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-delta 1 Catenin/CAS (phospho S268)抗体[EPR2380]
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔单克隆抗体[EPR2380] to delta 1 Catenin/CAS (phospho S268) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho S268).
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阳性对照
- HeLa cell lysate treated with Calyculin A. A431 cells. Human breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2380 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)All lanes : Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.cn/ps/products/79/ab79545/Images/ab79545-95093-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)Immunofluorescent staining of delta 1 Catenin/CAS in A431 cells using ab79545 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.cn/ps/products/79/ab79545/Images/ab79545-95094-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)ab79545 at 1/100 dilution staining delta 1 Catenin/CAS in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.cn/ps/products/79/ab79545/Images/ab79545-4-benefits-of-recombinant-antibodies.png)
Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab79545 被引用在 1 文献中.
- Alharatani R et al. Novel truncating mutations in CTNND1 cause a dominant craniofacial and cardiac syndrome. Hum Mol Genet 29:1900-1921 (2020). PubMed: 32196547