Anti-delta 1 Catenin/CAS抗体(ab72039)
Key features and details
- Rabbit polyclonal to delta 1 Catenin/CAS
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-delta 1 Catenin/CAS抗体
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔多克隆抗体to delta 1 Catenin/CAS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
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免疫原
Synthetic peptide corresponding to Human delta 1 Catenin/CAS aa 75-125.
Database link: NP_001322.1 -
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72039 was affinity purified using an epitope specific to delta 1 Catenin/CAS immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72039于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/1000.
Epitope retrieval with Tris-EDTA pH 9.0 is recommended. |
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| WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/200 - 1/1000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended. |
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WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Entrez Gene: 100519699 Pig
- Entrez Gene: 311163 Rat
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
see all -
别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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Western blot - Anti-delta 1 Catenin/CAS antibody (ab72039)All lanes : Anti-delta 1 Catenin/CAS antibody (ab72039) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody (ab72039)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling delta 1 Catenin/CAS with ab72039 at 1/1000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody (ab72039)Detection of delta 1 Catenin/CAS by Western Blot of Immunprecipitate.
ab72039 at 1µg/ml staining delta 1 Catenin/CAS in HeLa whole cell lysate immunoprecipitated using ab72039 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab72039 被引用在 1 文献中.
- Lenin R et al. GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability. Sci Rep 9:10783 (2019). PubMed: 31346222