重组Anti-DEF6抗体[EPR7492] (ab126792)
![Western blot - Anti-DEF6 antibody [EPR7492] (ab126792)](https://www.abcam.cn/ps/products/126/ab126792/Images/ab126792-140868-anti-def6-antibody-epr7492-western-blot.jpg "Western blot - Anti-DEF6 antibody [EPR7492] (ab126792)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7492] to DEF6
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DEF6抗体[EPR7492]
参阅全部 DEF6 一抗 -
描述
兔单克隆抗体[EPR7492] to DEF6 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human tonsil, Ramos, MOLT4, Human thymus, Raji and Jurkat lysates; permeabilized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.01 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7492 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126792于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
靶标
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功能
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling. -
组织特异性
Broadly expressed in the immune system and can be detected in T and B-cells. -
序列相似性
Contains 1 PH domain. -
结构域
The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. -
翻译后修饰
Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation. -
细胞定位
Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate. - Information by UniProt
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数据库链接
- Entrez Gene: 50619 Human
- Omim: 610094 Human
- SwissProt: Q9H4E7 Human
- Unigene: 15476 Human
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别名
- DEF-6 antibody
- Def6 antibody
- DEFI6_HUMAN antibody
see all
图片
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Western blot - Anti-DEF6 antibody [EPR7492] (ab126792)All lanes : Anti-DEF6 antibody [EPR7492] (ab126792) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Ramos lysate
Lane 3 : MOLT4 lysate
Lane 4 : Human thymus lysate
Lane 5 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa -
![Flow Cytometry (Intracellular) - Anti-DEF6 antibody [EPR7492] (ab126792)](https://www.abcam.cn/ps/products/126/ab126792/Images/ab126792-140867-anti-def6-antibody-epr7492-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-DEF6 antibody [EPR7492] (ab126792)ab126792 at 1/100 dilution staining DEF6 in permeabilized Jurkat cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).
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![OI-RD Scanning - Anti-DEF6 antibody [EPR7492] (ab126792)](https://www.abcam.cn/ps/products/126/ab126792/Images/ab126792-140866-anti-def6-antibody-epr7492.jpg)
OI-RD Scanning - Anti-DEF6 antibody [EPR7492] (ab126792) -
![Anti-DEF6 antibody [EPR7492] (ab126792)](https://www.abcam.cn/ps/products/126/ab126792/Images/ab126792-4-benefits-of-recombinant-antibodies.png)
Anti-DEF6 antibody [EPR7492] (ab126792)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126792 被引用在 1 文献中.
- Joshi RN et al. Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells. Front Immunol 8:1163 (2017). PubMed: 28993769