Anti-Dectin-1抗体[GE2] - Low endotoxin，Azide free (ab82888)
Key features and details
- Mouse monoclonal [GE2] to Dectin-1 - Low endotoxin, Azide free
- Suitable for: Inhibition Assay, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Dectin-1抗体[GE2] - Low endotoxin，Azide free
参阅全部 Dectin-1 一抗 -
描述
小鼠单克隆抗体[GE2] to Dectin-1 - Low endotoxin，Azide free -
宿主
Mouse -
特异性
Human beta Glucagon Receptor has two major (ßGR-A and ßGR-B) and several minor isoforms. ßGR-A and ßGR-B differ by the presence and absence, respectively, of a stalk region. ab82888 (clone GE2) recognizes both isoform A and B. -
经测试应用
适用于: Inhibition Assay, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Tissue, cells or virus corresponding to Human Dectin-1. NIH/3T3 cells expressing full length Dectin-1.
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阳性对照
- Human peripheral blood monocytes
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Endotoxin level: <0.01EU/ug -
克隆
单克隆 -
克隆编号
GE2 -
骨髓瘤
Sp2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82888于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Inhibition Assay |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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Inhibition Assay
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
组织特异性
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
疾病相关
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
序列相似性
Contains 1 C-type lectin domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to glucan binding. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 64581 Human
- Entrez Gene: 56644 Mouse
- Omim: 606264 Human
- SwissProt: Q9BXN2 Human
- SwissProt: Q6QLQ4 Mouse
- Unigene: 143929 Human
- Unigene: 239516 Mouse
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别名
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
数据表及文件
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Datasheet download
文献 (9)
ab82888 被引用在 9 文献中.
- Wang Y et al. Fungal dysbiosis of the gut microbiota is associated with colorectal cancer in Chinese patients. Am J Transl Res 13:11287-11301 (2021). PubMed: 34786058
- Keizer EM et al. EphA2-Dependent Internalization of A. fumigatus Conidia in A549 Lung Cells Is Modulated by DHN-Melanin. Front Microbiol 11:534118 (2020). PubMed: 33123097
- Camilli G et al. ß-Glucan-induced reprogramming of human macrophages inhibits NLRP3 inflammasome activation in cryopyrinopathies. J Clin Invest 130:4561-4573 (2020). PubMed: 32716363
- Camilli G et al. Impaired phagocytosis directs human monocyte activation in response to fungal derived ß-glucan particles. Eur J Immunol 48:757-770 (2018). PubMed: 29313961
- Xiao Y et al. Targeting CBLB as a potential therapeutic approach for disseminated candidiasis. Nat Med 22:906-14 (2016). PubMed: 27428899
- Bao Z et al. Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells. BMC Microbiol 15:161 (2015). PubMed: 26268695
- Kyrmizi I et al. Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling. J Immunol 191:1287-99 (2013). PubMed: 23817424
- Linden JR et al. The role of galectin-3 in phagocytosis of Candida albicans and Candida parapsilosis by human neutrophils. Cell Microbiol 15:1127-42 (2013). PubMed: 23279221
- Han X et al. ß-1,3-Glucan-Induced Host Phospholipase D Activation Is Involved in Aspergillus fumigatus Internalization into Type II Human Pneumocyte A549 Cells. PLoS One 6:e21468 (2011). Flow Cyt, Inhibition Assay ; Human . PubMed: 21760893
