Anti-Decorin抗体(ab175404)
Key features and details
- Rabbit polyclonal to Decorin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Decorin抗体
参阅全部 Decorin 一抗 -
描述
兔多克隆抗体to Decorin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Decorin aa 31-359.
Sequence:DEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLP PDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVK LERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIE LGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDG NKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNK LTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLF SNPVQYWEIQPSTFRCVYVRSAIQLGNYK
Database link: P07585 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.91% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175404于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
靶标
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功能
May affect the rate of fibrils formation. -
疾病相关
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
翻译后修饰
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1634 Human
- Entrez Gene: 13179 Mouse
- Omim: 125255 Human
- SwissProt: P07585 Human
- SwissProt: P28654 Mouse
- Unigene: 728830 Human
- Unigene: 56769 Mouse
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别名
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
see all
图片
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Western blot - Anti-Decorin antibody (ab175404)All lanes : Anti-Decorin antibody (ab175404) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDaBlocking buffer: 3% nonfat dry milk in TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-Decorin antibody (ab175404)Immunofluorescence analysis of MCF-7 (human breast adenocarcinoma cell line) cells stained for Decorin (green) using ab175404.
DAPI used for nuclear staining (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab175404 被引用在 20 文献中.
- Strokotova AV et al. Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats. Biomed Rep 20:7 (2024). PubMed: 38124768
- Chen P et al. Single-Cell and Spatial Transcriptomics Decodes Wharton's Jelly-Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures. Adv Sci (Weinh) 10:e2204786 (2023). PubMed: 36504438
- Busto F et al. Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging. Molecules 28:N/A (2023). PubMed: 37298828
- Rampin A et al. Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture. Cells 11:N/A (2022). PubMed: 35563866
- Best KT et al. Scleraxis-lineage cell depletion improves tendon healing and disrupts adult tendon homeostasis. Elife 10:N/A (2021). PubMed: 33480357