Anti-DDX6抗体(ab70455)
Key features and details
- Rabbit polyclonal to DDX6
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DDX6抗体
参阅全部 DDX6 一抗 -
描述
兔多克隆抗体to DDX6 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a sequence between N terminal residues 1 to 50 of human DDX6 (Swiss-Prot P26196).
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阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70455 was affinity purified using an epitope specific to DDX6 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB | (2) |
1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. -
细胞定位
Cytoplasm; P-body. Note: Processing bodies (PB). -
数据库链接
- Entrez Gene: 419783 Chicken
- Entrez Gene: 513906 Cow
- Entrez Gene: 100135517 Guinea pig
- Entrez Gene: 1656 Human
- Entrez Gene: 13209 Mouse
- Entrez Gene: 100190811 Orangutan
- Entrez Gene: 500988 Rat
- Omim: 600326 Human
see all -
别名
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DDX6 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab70455)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DDX6 with ab70455 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-DDX6 antibody (ab70455)All lanes : Anti-DDX6 antibody (ab70455) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunoprecipitation - Anti-DDX6 antibody (ab70455)250µg HeLa whole cell lysate treated with ab70455 at 0.04 µg/ml for the Western blot, and at 3 µg/mg lysate for Immunoprecipitation.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70455 被引用在 2 文献中.
- Wilczynska A et al. eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR. Genome Biol 20:262 (2019). PubMed: 31791371
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708