AB109515

重组Anti-DDX50抗体[EPR5273]

Anti-DDX50 antibody [EPR5273]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal DDX50 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

查看别名

ATP-dependent RNA helicase DDX50, DEAD box protein 50, Gu-beta, Nucleolar protein Gu2, DDX50

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX50 antibody [EPR5273] (AB109515)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX50 antibody [EPR5273] (AB109515)

Immunohistochemical analysis of DDX50 in paraffin embedded Human thyroid gland adenocarcinoma tissue using ab109515 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-DDX50 antibody [EPR5273] (AB109515)

All lanes:

Western blot - Anti-DDX50 antibody [EPR5273] (ab109515) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-DDX50 antibody [EPR5273] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5273

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DDX50 also known as DEAD box polypeptide 50 is an RNA helicase with ATPase activity that unwinds RNA functioning in ribosome biogenesis. It belongs to the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (D-E-A-D). The molecular weight of DDX50 is approximately 82 kDa. This protein expresses mainly in nucleoli within cells where it likely participates in pre-rRNA processing and ribosomal subunit assembly.

Biological function summary

The protein DDX50 plays roles in RNA processing and modification during ribosome production. It does not function alone but as part of a ribonucleoprotein complex that facilitates the maturation of ribosomal RNA. By interacting with other components it assists in the structural rearrangements and modifications necessary for proper ribosome assembly impacting overall cellular protein synthesis.

Pathways

Cellular processes involving ribosome assembly and function depend on proteins like DDX50. It integrates in the ribosome biogenesis pathway influencing the synthesis and assembly of ribosomal subunits. In this pathway DDX50 works with proteins such as DDX21 and NOP56 sharing functional domains to facilitate efficient rRNA modification and processing which are critical to ribosome construction and cellular growth.

Alterations in DDX50 expression or function connect to cancer and some hematological conditions. DDX50's role in ribosome biogenesis makes it a factor in tumorigenesis particularly in cancers where cell proliferation relies heavily on increased protein synthesis. Additionally DDX50 interaction with proteins like NOP56 points to a potential link to dyskeratosis congenita a disorder affecting bone marrow and the skin. Understanding these connections may open doors for developing diagnostic or therapeutic strategies targeting DDX50 and similar proteins.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information DDX50

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36978950

2023

Different Effects and Mechanisms of Selenium Compounds in Improving Pathology in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Hao Zhang,Jia-Ying Peng,Yu-Bin Chen,Chao Wang,Chen Chen,Guo-Li Song

Viruses 14: PubMed35215908

2022

DDX50 Is a Viral Restriction Factor That Enhances IRF3 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mitchell A Pallett,Yongxu Lu,Geoffrey L Smith

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-DDX50抗体[EPR5273]