Anti-DDX5 (phospho Y593)抗体(ab62255)
Key features and details
- Rabbit polyclonal to DDX5 (phospho Y593)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX5 (phospho Y593)抗体
参阅全部 DDX5 一抗 -
描述
兔多克隆抗体to DDX5 (phospho Y593) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human DDX5 aa 550-650 (phospho Y593).
Database link: P17844 -
阳性对照
- ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
序列相似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Arg-502 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
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别名
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-DDX5 (phospho Y593) antibody (ab62255)ab62255 at 1/500 - 1/1000 dilution staining DDX5 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab62255 被引用在 2 文献中.
- Zhao X et al. Long Noncoding RNA NHEG1 Drives ß-Catenin Transactivation and Neuroblastoma Progression through Interacting with DDX5. Mol Ther 28:946-962 (2020). PubMed: 31982037
- Arun G et al. mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells. Mol Cell Biol 32:3140-52 (2012). PubMed: 22665494