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使用敲除细胞株进行验证重组RabMAb

重组Anti-DDX5抗体[EPR7240] (ab128928)

Submit a review Submit a question References (2)
Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)
  • Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)
  • Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7240] (ab128928)
  • Anti-DDX5 antibody [EPR7240] (ab128928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7240] to DDX5
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-DDX5抗体[EPR7240]
    参阅全部 DDX5 一抗

  • 描述

    兔单克隆抗体[EPR7240] to DDX5

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide within Human DDX5 aa 550-650. The exact sequence is proprietary.

  • 阳性对照

    • HT29 and SHSY5Y cell lysates; Human breast carcinoma and colon tissues; SHSY5Y cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR7240

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab128928于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.

  • 序列相似性

    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • 翻译后修饰

    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.

  • 细胞定位

    Nucleus > nucleolus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ATP dependent RNA helicase DDX5 antibody
      • DDX 5 antibody
      • Ddx5 antibody
      • DDX5_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
      • DEAD box 5 antibody
      • DEAD box protein 5 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
      • G17P1 antibody
      • HELR antibody
      • HLR1 antibody
      • HUMP68 antibody
      • P68 antibody
      • p68 RNA helicase antibody
      • Probable ATP dependent RNA helicase DDX5 antibody
      • Probable ATP-dependent RNA helicase DDX5 antibody
      • RNA helicase p68 antibody
      see all

    图片

    • Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)
      Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab128928 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab128928 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab128928 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)
      Western blot - Anti-DDX5 antibody [EPR7240] (ab128928)
      All lanes : Anti-DDX5 antibody [EPR7240] (ab128928) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 69 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)

      ab128928 at 1/100 dilution staining DDX5 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7240] (ab128928)

      ab128928 at 1/100 dilution staining DDX5 in paraffin-embedded Human colon tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7240] (ab128928)
      Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7240] (ab128928)
      ab128928 at 1/100 dilution staining DDX5 in SHSY5Y cells by immunofluorescence.
    • Anti-DDX5 antibody [EPR7240] (ab128928)
      Anti-DDX5 antibody [EPR7240] (ab128928)

    文献 (2)

    发表研究结果有使用 ab128928？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab128928 被引用在 2 文献中.

    • Yan P  et al. LncRNA Platr22 promotes super-enhancer activity and stem cell pluripotency. J Mol Cell Biol 13:295-313 (2021). PubMed: 33049031
    • Jiang FZ  et al. Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing. Oncotarget 6:44660-74 (2015). IP . PubMed: 26587974

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