Anti-DDX5抗体(ab21696)
Key features and details
- Rabbit polyclonal to DDX5
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DDX5抗体
参阅全部 DDX5 一抗 -
描述
兔多克隆抗体to DDX5 -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab21696 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Product promise guarantee. You may also be interested in our alternative recombinant antibody, ab126730.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human DDX5 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab22412) -
阳性对照
- This antibody gave a positive signal in the following lysates: Hela nuclear lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21696于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
序列相似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Arg-502 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Entrez Gene: 287765 Rat
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
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别名
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
图片
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Western blot - Anti-DDX5 antibody (ab21696)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab21696 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab21696 was shown to specifically react with DDX5 in wild-type HAP1 cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab21696 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody (ab21696)ab21696 staining DDX5 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21696 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-DDX5 antibody (ab21696)ab21696 recognizes a clear, strong band at ~ 69kDa corresponding to DDX5. A number of other, non specific bands are weakly recognized by the antibody. All bands are quenched by the addition of the blocking peptide.
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Western blot - Anti-DDX5 antibody (ab21696)All lanes : Anti-DDX5 antibody (ab21696) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab21696 被引用在 33 文献中.
- Prabhakar A et al. Essential role of the amino-terminal region of Drosha for the Microprocessor function. iScience 26:107971 (2023). PubMed: 37810246
- Islam Z et al. Active enhancers strengthen insulation by RNA-mediated CTCF binding at chromatin domain boundaries. Genome Res 33:1-17 (2023). PubMed: 36650052
- Hu T et al. Circular RNA PUM1 performs as a competing endogenous RNA of microRNA-340-5p to mediate DEAD-box helicase 5 to mitigate cerebral ischemia-reperfusion injury. Bioengineered 13:11564-11578 (2022). PubMed: 35510394
- So KKH et al. seRNA PAM controls skeletal muscle satellite cell proliferation and aging through trans regulation of Timp2 expression synergistically with Ddx5. Aging Cell 21:e13673 (2022). PubMed: 35851988
- Shallak M et al. The endogenous HBZ interactome in ATL leukemic cells reveals an unprecedented complexity of host interacting partners involved in RNA splicing. Front Immunol 13:939863 (2022). PubMed: 35979358