Anti-DDX5抗体(ab10261)
Key features and details
- Goat polyclonal to DDX5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DDX5抗体
参阅全部 DDX5 一抗 -
描述
山羊多克隆抗体to DDX5 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human DDX5 aa 600 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: HeLa, A431, HepG2, Jurkat, and NIH3T3 nuclear lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
序列相似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Arg-502 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
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别名
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
图片
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Western blot - Anti-DDX5 antibody (ab10261)All lanes : Anti-DDX5 antibody (ab10261) at 0.5 µg/ml
Lane 1 : A431 cell nuclear lysate
Lane 2 : HeLa cell nuclear lysate
Lane 3 : HepG2 cell nuclear lysate
Lane 4 : Jurkat cell nuclear lysate
Lane 5 : NIH3T3 cell nuclear lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 69 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab10261 被引用在 25 文献中.
- Polvèche H et al. SplicingLore: a web resource for studying the regulation of cassette exons by human splicing factors. Database (Oxford) 2023:N/A (2023). PubMed: 38128543
- Terrone S et al. RNA helicase-dependent gene looping impacts messenger RNA processing. Nucleic Acids Res 50:9226-9246 (2022). PubMed: 36039747
- Gualtieri A et al. The RNA helicase DDX5 cooperates with EHMT2 to sustain alveolar rhabdomyosarcoma growth. Cell Rep 40:111267 (2022). PubMed: 36044855
- Sessa G et al. BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair‡. EMBO J 40:e106018 (2021). PubMed: 33634895
- Dixon CR et al. STING nuclear partners contribute to innate immune signaling responses. iScience 24:103055 (2021). PubMed: 34541469