Anti-DDX47抗体(ab225870)
Key features and details
- Rabbit polyclonal to DDX47
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DDX47抗体
参阅全部 DDX47 一抗 -
描述
兔多克隆抗体to DDX47 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster
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免疫原
Synthetic peptide within Human DDX47 aa 405-455. The exact sequence is proprietary. (NP_057439.2).
Database link: Q9H0S4 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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序列相似性
Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 465298 Chimpanzee
- Entrez Gene: 100751146 Chinese hamster
- Entrez Gene: 534721 Cow
- Entrez Gene: 102119574 Cynomolgus monkey
- Entrez Gene: 101148379 Gorilla
- Entrez Gene: 100063569 Horse
- Entrez Gene: 51202 Human
- Entrez Gene: 67755 Mouse
see all -
别名
- DDX47 antibody
- DDX47_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 47 antibody
see all
图片
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Western blot - Anti-DDX47 antibody (ab225870)All lanes : Anti-DDX47 antibody (ab225870) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-DDX47 antibody (ab225870)DDX47 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225870 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225870 at 0.4 µg/ml.
Lane 1: ab225870 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225870 尚未被引用在任何文献中。