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重组RabMAb

重组Anti-DDX39抗体[EPR13508] (ab180857)

Reviews (1) Submit a question References (1)
Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)
  • Flow Cytometry (Intracellular) - Anti-DDX39 antibody [EPR13508] (ab180857)
  • Anti-DDX39 antibody [EPR13508] (ab180857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13508] to DDX39
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-DDX39抗体[EPR13508]
    参阅全部 DDX39 一抗

  • 描述

    兔单克隆抗体[EPR13508] to DDX39

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WBmore details
    不适用于: ICC/IF or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human DDX39 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O00148

  • 阳性对照

    • Jurkat, A431, HeLa, HepG2, and Human seminoma lysates. Human breast and colon tissue. Permabilized Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180857于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
应用说明
Is unsuitable for ICC/IF or IP.

靶标

  • 相关性

    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.

  • 细胞定位

    Cytoplasmic and Nuclear

  • 数据库链接

    • 别名

      • ATP dependent RNA helicase DDX39 antibody
      • ATP-dependent RNA helicase DDX39A antibody
      • BAT1 antibody
      • BAT1L antibody
      • DDX39 antibody
      • DDXL antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
      • DEAD box protein 39 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
      • Eukaryotic initiation factor 4a antibody
      • Nuclear RNA helicase URH49 antibody
      • Nuclear RNA helicase, DECD variant of DEAD box family antibody
      • UAP56 related helicase antibody
      • UAP56 related helicase, 49 kDa antibody
      • URH49 antibody
      • wu:fc87b12 antibody
      • zgc:55881 antibody
      see all

    图片

    • Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)
      Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)
      All lanes : Anti-DDX39 antibody [EPR13508] (ab180857) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Human seminoma tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)

      Immunohistochemical analysis of Human breast tissue, staining DDX39 with ab180857 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)

      Immunohistochemical analysis of Human colon tissue, staining DDX39 with ab180857 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-DDX39 antibody [EPR13508] (ab180857)
      Flow Cytometry (Intracellular) - Anti-DDX39 antibody [EPR13508] (ab180857)

      Intracellular Flow Cytometry analysis of DDX39 expression in permeabilized Jurkat cells usingab180857 at 1/10 dilution (red) and a rabbit IgG as negative control (green). 

       

       

    • Anti-DDX39 antibody [EPR13508] (ab180857)
      Anti-DDX39 antibody [EPR13508] (ab180857)

    文献 (1)

    发表研究结果有使用 ab180857？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab180857 被引用在 1 文献中.

    • Dobrotkova V  et al. Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers. PLoS One 14:e0218269 (2019). PubMed: 31188873

    客户评价及客户问答

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    Western blot abreview for Anti-DDX39 antibody [EPR13508]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    25 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Robert Kupp

    Verified customer

    提交于 Oct 20 2020

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