AB70821

Anti-DDX28抗体

Name: DDX28抗体 (ab70821)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70821
Price: 4606 CNY
Availability: InStock

Anti-DDX28 antibody

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(4 Publications)

Rabbit Polyclonal DDX28 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DDX28.

查看别名

MDDX28, DDX28, Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28

2 Images

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Immunoprecipitation - Anti-DDX28 antibody (AB70821)

Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-DDX28 antibody (ab70821)

Predicted band size: 60 kDa

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Unknown

Western blot - Anti-DDX28 antibody (AB70821)

All lanes:

Western blot - Anti-DDX28 antibody (ab70821) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 60 kDa

Observed band size: 28 kDa,60 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human DDX28. The exact immunogen used to generate this antibody is proprietary information.

Q9NUL7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The DDX28 protein also known as DEAD box helicase 28 is a member of the DEAD box protein family. Its molecular mass is approximately 57 kDa. This family of proteins is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX28 functions as an ATP-dependent RNA helicase which means it unwinds RNA structures and plays roles in rearranging ribonucleoprotein complexes. This protein is expressed in several tissues with significant expression in the liver and heart with the potential for further tissue-specific investigations.

Biological function summary

DDX28 participates in RNA processing and mitochondrial ribosome biogenesis activities. It is known to be a component of the mitoribosome a specialized ribosome for mitochondria. Being part of the mitoribosome DDX28 assists in the synthesis and assembly of proteins that are encoded by the mitochondrial genome. As helicases generally support the proper assembly and function of these complexes DDX28's role becomes important for maintaining mitochondrial translation integrity and efficiency.

Pathways

DDX28 interacts with several components within mitochondrial translation and RNA processing pathways. It associates notably with other mitochondrial ribosomal proteins forming a connection to the larger mitochondrial protein synthesis and assembly pathway. This connection is essential for the translation of mitochondrially-encoded proteins. DDX28 also has potential links with other helicase proteins such as DDX3X which leads to the interplay within various RNA helicase-involved pathways contributing to RNA metabolism and homeostasis in cells.

Mutations or dysregulation of DDX28 have been connected to mitochondrial dysfunction disorders including mitochondrial biogenesis deficiencies which impacts cellular energy homeostasis and can lead to serious conditions. Its involvement with other helicases such as DHX30 is also significant in certain neurodegenerative disorders. These associations highlight how alterations in DDX28's function can significantly affect cellular physiology and cascade into disease states when mitochondrial protein synthesis is impaired.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed : 25683708, PubMed : 25683715). May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity (PubMed : 11350955).

See full target information DDX28

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:166 PubMed38388496

2024

Alternative Wnt-signaling axis leads to a break of oncogene-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Viola Kluge,Melanie Kappelmann-Fenzl,Stefan Fischer,Tom Zimmermann,Michaela Pommer,Silke Kuphal,Anja-Katrin Bosserhoff

PLoS genetics 16:e1008923 PubMed32735630

2020

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Paola Favia,Sara Vidoni,Vittoria Petruzzella,Massimo Zeviani

Molecular and cellular biology 40: PubMed31907278

2020

DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia L Evagelou,Olivia Bebenek,Erin J Specker,James Uniacke

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani

View all publications

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Anti-DDX28抗体

Anti-DDX28 antibody

Immunoprecipitation - Anti-DDX28 antibody (AB70821)

Western blot - Anti-DDX28 antibody (AB70821)