AB70463

Anti-DDX24 抗体

Name: DDX24抗体 (ab70463)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70463
Price: 4606 CNY
Availability: InStock

Anti-DDX24 antibody

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(3 Publications)

Rabbit Polyclonal DDX24 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX24.

查看别名

ATP-dependent RNA helicase DDX24, DEAD box protein 24, DDX24

2 Images

Supplier Data

Immunoprecipitation - Anti-DDX24 antibody (AB70463)

DDX24 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70463 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70463 at 1 μg/mL.

Lane 1 : ab70463 IP in HeLa whole cell lysate.

Lane 2 : ab70463 IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-DDX24 antibody (ab70463)

Predicted band size: 96 kDa

false

Supplier Data

Western blot - Anti-DDX24 antibody (AB70463)

All lanes:

Western blot - Anti-DDX24 antibody (ab70463) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 96 kDa

false

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human DDX24. The exact immunogen used to generate this antibody is proprietary information.

Q9GZR7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DDX24 also known as DEAD-box helicase 24 is a member of the DEAD box protein family and functions as an ATP-dependent RNA helicase. This protein has a molecular mass of approximately 91 kDa. It is expressed in various tissues with notable levels seen in testis and heart. The expression pattern indicates its involvement in cellular processes that require tight regulation of RNA metabolism.

Biological function summary

DEAD-box helicases like DDX24 are important in RNA processing and ribosome biogenesis. DDX24 contributes to pre-rRNA processing by participating in the production and assembly of ribosomal subunits. The protein is also a part of the ribonucleoprotein complex where it influences the regulation of RNA interference processes. This suggests its essential role in RNA stability and gene expression regulation.

Pathways

DDX24 plays a significant role in the ribosome biogenesis pathway and RNA processing pathways. Both are fundamental for efficient protein synthesis. Within these pathways DDX24 interacts with other proteins like NOP56 and fibrillarin which are influential in the process of rRNA modification and folding. Such interactions are vital for the proper assembly of ribosomal subunits and their correct function in protein translation.

Dysregulation of DDX24 expression or function has been linked to certain cancers such as brain tumors where alterations in RNA processing are frequent. Moreover irregular DDX24 activity has been associated with viral infections such as HIV where the virus exploits host RNA processing machineries. This connection includes proteins like DDX6 which work with DDX24 during the viral replication cycle affecting the host's cellular environment and promoting disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ATP-dependent RNA helicase.

See full target information DDX24

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 19:596-608 PubMed35370459

2022

Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglin Li,Xiaoyun Chen,Jiebing Gao,Jianzhong Xian,Zhijun Li,Lei Bi,Min Yang,Shuai Yang,Hongjun Jin,Hong Shan

Molecular & cellular proteomics : MCP 20:100142 PubMed34478875

2021

Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Wu,Jennifer Nance,Feixia Chu,Thomas G Fazzio

Nature communications 8:15615 PubMed28561026

2017

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Cloutier,Christian Poitras,Mathieu Durand,Omid Hekmat,Émilie Fiola-Masson,Annie Bouchard,Denis Faubert,Benoit Chabot,Benoit Coulombe

View all publications

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Anti-DDX24 抗体

Anti-DDX24 antibody

Immunoprecipitation - Anti-DDX24 antibody (AB70463)

Western blot - Anti-DDX24 antibody (AB70463)