AB228999

Anti-DDX23 抗体

Name: DDX23抗体 (ab228999)| Abcam中文官网
Brand: Abcam Limited
SKU: AB228999
Price: 4748 CNY
Availability: InStock

Anti-DDX23 antibody

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(1 Publication)

Rabbit Polyclonal DDX23 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX23.

查看别名

Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX23 antibody (AB228999)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX23 antibody (AB228999)

Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) cells stained for DDX23 protein using ab228999 at 1/500 dilution in ICC/IF.

Alpha-tubulin filaments (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB228999)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB228999)

Paraffin-embedded HBL435 xenograft tissue stained for DDX23 protein using ab228999 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DDX23 antibody (AB228999)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DDX23 antibody (ab228999) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 96 kDa

true

Supplier Data

Western blot - Anti-DDX23 antibody (AB228999)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DDX23 antibody (ab228999) at 1/1000 dilution

Lane 1:

NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

JC (Mouse mammary gland cell line) whole cell lysate at 30 µg

Predicted band size: 96 kDa

true

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human DDX23. The exact immunogen used to generate this antibody is proprietary information.

Q9BUQ8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The DDX23 protein also known as DEAD-box helicase 23 is a member of the DEAD-box protein family. This family is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and is involved in a wide range of cellular processes. DDX23 plays a mechanical role in unwinding RNA which is essential for RNA processing. The protein has a molecular mass of approximately 82 kDa. DDX23 is expressed ubiquitously across various tissues with significant expression in the nucleus where it contributes to RNA dynamics.

Biological function summary

DEAD-box helicase 23 participates in the spliceosome complex important for pre-mRNA splicing a process that removes introns and joins exons to form mature messenger RNA. DDX23 acts during spliceosome assembly and the catalytic activation phase. Through its helicase activity it facilitates structural transitions within the spliceosome ensuring accurate splicing of pre-mRNA molecules. This function is essential for accurate gene expression and cellular function.

Pathways

DDX23 is involved in the spliceosomal pathway and related RNA processing pathways. Its role in pre-mRNA splicing links DDX23 to the regulation of gene expression impacting numerous downstream pathways. Within these pathways DDX23 interacts closely with other spliceosome-associated proteins like PRPF8 and SNRNP200. These interactions ensure the efficiency and regulation of the splicing process critical to cellular and physiological functions.

Alterations in the DDX23 function have been associated with conditions such as cancers and neurodegenerative diseases. Misregulation of DDX23 activity can lead to aberrant splicing events contributing to oncogenesis. Additionally DDX23 abnormalities correlate with neurodegenerative conditions where correct RNA processing is disrupted. In these disorders DDX23 interfaces with proteins such as PRPF8 highlighting its importance in maintaining cellular and organismal health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation (PubMed : 18425142). Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 28076779).

See full target information DDX23

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35055180

2022

Biallelic Variants in Cause a Severe Infantile Metabolic Disorder Affecting Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole J Van Bergen,Daniella H Hock,Lucy Spencer,Sean Massey,Tegan Stait,Zornitza Stark,Sebastian Lunke,Ain Roesley,Heidi Peters,Joy Yaplito Lee,Anna Le Fevre,Oliver Heath,Cristina Mignone,Joseph Yuan-Mou Yang,Monique M Ryan,Colleen D'Arcy,Margot Nash,Sile Smith,Nikeisha J Caruana,David R Thorburn,David A Stroud,Susan M White,John Christodoulou,Natasha J Brown

View all publications

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Anti-DDX23 抗体

Anti-DDX23 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DDX23 antibody (AB228999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB228999)

Western blot - Anti-DDX23 antibody (AB228999)

Western blot - Anti-DDX23 antibody (AB228999)