重组Anti-DDX21抗体[EPR14495] (ab182156)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14495] to DDX21
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-DDX21抗体[EPR14495]
参阅全部 DDX21 一抗 -
描述
兔单克隆抗体[EPR14495] to DDX21 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, and Jurkat lysates. IHC-P: human breast carcinoma tissue. ICC/IF: HeLa cells and HepG2 cells. Flow Cyt (intra): HepG2 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14495 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182156于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/60.
|
|
ICC/IF |
1/500.
|
|
IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100 |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/60. |
ICC/IF
1/500. |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100 |
WB
1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
靶标
-
功能
Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing. -
序列相似性
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 9188 Human
- Omim: 606357 Human
- SwissProt: Q9NR30 Human
- Unigene: 223141 Human
-
别名
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1:200 dilution (2.88 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling DDX21 with Purified ab182156 at 1:200 dilution (2.89 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1/60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
All lanes : Anti-DDX21 antibody [EPR14495] (ab182156) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa -
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue, staining DDX21 with ab182156 at 1/100 dilution. Hematoxylin was used as a counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunofluorescence analysis of HeLa cells, staining DDX21 (red) with ab182156 at 1/100 dilution. An Alexa Fluor®555 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed with -20℃ Acetone. Nuclei were counterstained with DAPI (blue).
-
Immunofluorescence analysis of HepG2 cells, staining DDX21 (green) with ab182156 at 1/100 dilution. An Alexa Fluor®488 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed in 4% paraformaldehyde. Nuclei were counterstained with DAPI (blue).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (6)
ab182156 被引用在 6 文献中.
- Drino A et al. Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res 51:1326-1352 (2023). PubMed: 36718960
- Zhou J et al. Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein. Front Microbiol 13:802740 (2022). PubMed: 35283818
- Wu W et al. Caspase-Dependent Cleavage of DDX21 Suppresses Host Innate Immunity. mBio 12:e0100521 (2021). PubMed: 34125604
- Xu W et al. Systematic Genome-Wide Profiles Reveal Alternative Splicing Landscape and Implications of Splicing Regulator DExD-Box Helicase 21 in Aggressive Progression of Adrenocortical Carcinoma. Phenomics 1:243-256 (2021). PubMed: 36939770
- Johansson JA et al. PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation. Dev Cell 54:317-332.e9 (2020). PubMed: 32652076
- Misiewicz-Krzeminska I et al. A novel nano-immunoassay method for quantification of proteins from CD138-purified myeloma cells: biological and clinical utility. Haematologica 103:880-889 (2018). PubMed: 29545347