Anti-DDX21抗体(ab126968)
Key features and details
- Rabbit polyclonal to DDX21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-DDX21抗体
参阅全部 DDX21 一抗 -
描述
兔多克隆抗体to DDX21 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant fragment, corresponding to a region within amino acids 101-300 of Human DDX21 (Q9NR30).
-
阳性对照
- A549, HeLa, HepG2 and HCT116 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
靶标
-
功能
Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing. -
序列相似性
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 781917 Cow
- Entrez Gene: 9188 Human
- Entrez Gene: 56200 Mouse
- Entrez Gene: 317399 Rat
- Omim: 606357 Human
- SwissProt: Q9NR30 Human
- SwissProt: Q9JIK5 Mouse
- SwissProt: Q3B8Q1 Rat
see all -
别名
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
see all
图片
-
Western blot - Anti-DDX21 antibody (ab126968)All lanes : Anti-DDX21 antibody (ab126968) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
7.5% SDS PAGE
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab126968 被引用在 3 文献中.
- Gapud EJ et al. Granzyme B Induces IRF-3 Phosphorylation through a Perforin-Independent Proteolysis-Dependent Signaling Cascade without Inducing Cell Death. J Immunol 206:335-344 (2021). PubMed: 33288544
- Patil A et al. Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function. J Virol 95:N/A (2021). PubMed: 33408177
- Cai Y et al. Broad Susceptibility of Nucleolar Proteins and Autoantigens to Complement C1 Protease Degradation. J Immunol 199:3981-3990 (2017). PubMed: 29070672