AB128197

Anti-DDX18抗体

Name: DDX18抗体 (ab128197)| Abcam中文官网
Brand: Abcam Limited
SKU: AB128197
Price: 4606 CNY
Availability: InStock

Anti-DDX18 antibody

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(3 Publications)

Rabbit Polyclonal DDX18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DDX18.

查看别名

cPERP-D, DDX18, ATP-dependent RNA helicase DDX18, DEAD box protein 18, Myc-regulated DEAD box protein, MrDb

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (AB128197)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (AB128197)

ab128197, at 1/500, staing of DDX18 in HeLa cells followed by FITC-conjugated anti-rabbit IgG second antibody by immunohistochemistry. DNA was stained with Hoechst dye.

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Western blot - Anti-DDX18 antibody (AB128197)

All lanes:

Western blot - Anti-DDX18 antibody (ab128197) at 1/1000 dilution

All lanes:

HeLa whole cell extract at 10 µg

Predicted band size: 75 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human DDX18. The exact immunogen used to generate this antibody is proprietary information.

Q9NVP1

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯度

Whole antiserum

存储溶液

pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: 99% Whole serum

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DDX18 also known as DEAD-box helicase 18 functions as an RNA helicase involved in the modification of RNA structures. It unwinds RNA and is important in ribosome biogenesis. The molecular weight of DDX18 is approximately 73 kDa. DDX18 is expressed in various tissues with significant presence in the nucleolus of cells where ribosome production occurs.

Biological function summary

DDX18 plays a role in the assembly and maturation of pre-ribosomal particles. It functions within a multi-protein complex and interacts with other nucleolar proteins. This interaction is necessary for the conversion of pre-rRNA into mature rRNA which forms the structural and functional components of ribosomes essential for protein synthesis.

Pathways

DDX18 participates in the ribosome biogenesis pathway which is key to cellular protein production. It is involved in the nucleolar preribosome process alongside proteins like NOP58 and fibrillarin. DDX18 contributes to the processing of pre-rRNA intermediates critical for creating functional ribosomes which are necessary for cell growth and proliferation.

Mutations or dysregulation of DDX18 associate with certain cancers like colorectal cancer. These changes impact ribosomal RNA processing and consequently protein synthesis leading to uncontrolled cell growth. DDX18's interaction with c-Myc a protein involved in cell cycle regulation and growth highlights its connection to cancer pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probable RNA-dependent helicase.

See full target information DDX18

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 16:1622-1634 PubMed40950356

2025

DDX18 promotes growth and metastasis of hepatocellular carcinoma via activating EMT and MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Xu,Xiaoxia Yu,Nannan Zhang,Feiran Wang,Zhong Chen

Cell reports 30:81-97.e7 PubMed31914400

2020

DEAD-Box Helicase 18 Counteracts PRC2 to Safeguard Ribosomal DNA in Pluripotency Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Zhongyang Wu,J Yuyang Lu,Bo Huang,Hongwei Zhou,Wei Xie,Jianlong Wang,Xiaohua Shen

Genome research 28:1405-1414 PubMed30108179

2018

Human proteins that interact with RNA/DNA hybrids.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel X Wang,Christopher Grunseich,Jennifer Fox,Joshua Burdick,Zhengwei Zhu,Niema Ravazian,Markus Hafner,Vivian G Cheung

View all publications

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Anti-DDX18抗体

Anti-DDX18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (AB128197)

Western blot - Anti-DDX18 antibody (AB128197)