Anti-DDX17抗体(ab70184)
Key features and details
- Rabbit polyclonal to DDX17
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DDX17抗体
参阅全部 DDX17 一抗 -
描述
兔多克隆抗体to DDX17 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to a region between C terminal residues 550 and 613 of human DDX17
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阳性对照
- WB: Whole cell lysate from HeLa, TCMK-1, 4T1, CT26.WT, C6; IHC: human ovarian carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70184 was affinity purified using an epitope specific to DDX17 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/15000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/15000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
靶标
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功能
RNA-dependent ATPase activity. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10521 Human
- Entrez Gene: 67040 Mouse
- Entrez Gene: 315133 Rat
- Omim: 608469 Human
- SwissProt: Q92841 Human
- SwissProt: Q501J6 Mouse
- Unigene: 528305 Human
- Unigene: 725500 Human
see all -
别名
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
see all
图片
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Western blot - Anti-DDX17 antibody (ab70184)All lanes : Anti-DDX17 antibody (ab70184) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Predicted band size: 72 kDa
Exposure time: 1 second -
Western blot - Anti-DDX17 antibody (ab70184)All lanes : Anti-DDX17 antibody (ab70184) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 4 : CT26.WT (Murine Colon Carcinoma) whole cell lysate
Lane 5 : C6 (rat glioma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 72 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody (ab70184)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DDX17 with ab70184 at 1/1000 (1µg/ml). Detection: DAB.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70184 被引用在 3 文献中.
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Motiño O et al. Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin Signaling. Mol Cell Biol 35:2554-67 (2015). WB . PubMed: 25963660
- Zaccara S et al. p53-directed translational control can shape and expand the universe of p53 target genes. Cell Death Differ N/A:N/A (2014). PubMed: 24926617