Anti-DDX17抗体[2248C2a] (ab71958)
![Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)](https://www.abcam.cn/ps/products/71/ab71958/Images/ab71958-353454-antiddx17antibody2248c2awesternblotknockouthek293ddx17helahumanlysates.jpg "Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)")
Key features and details
- Mouse monoclonal [2248C2a] to DDX17
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-DDX17抗体[2248C2a]
参阅全部 DDX17 一抗 -
描述
小鼠单克隆抗体[2248C2a] to DDX17 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment of human DDX17
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阳性对照
- Recombinant human DDX17. HEK-293 and HeLa cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab71958 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2248C2a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 33 kDa (predicted molecular weight: 72 kDa).
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 33 kDa (predicted molecular weight: 72 kDa). |
靶标
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功能
RNA-dependent ATPase activity. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10521 Human
- Omim: 608469 Human
- SwissProt: Q92841 Human
- Unigene: 528305 Human
- Unigene: 725500 Human
- Unigene: 727678 Human
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别名
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
see all
图片
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Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)All lanes : Anti-DDX17 antibody [2248C2a] (ab71958) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : DDX17 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 72 kDaLanes 1 - 3: Merged signal (red and green). Green - ab71958 observed at 72, 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71958 was shown to recognize DDX17 in wild-type HEK-293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab71958 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)](https://www.abcam.cn/ps/products/71/ab71958/Images/ab71958-86996-anti-ddx17-antibody-2248c2a-western-blot.gif)
Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)Anti-DDX17 antibody [2248C2a] (ab71958) + immunising recombinant protein
Predicted band size: 72 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
数据表及文件
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Datasheet download
文献 (2)
ab71958 被引用在 2 文献中.
- Musiani D et al. PRMT1 Is Recruited via DNA-PK to Chromatin Where It Sustains the Senescence-Associated Secretory Phenotype in Response to Cisplatin. Cell Rep 30:1208-1222.e9 (2020). PubMed: 31995759
- Lambert MP et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res 46:7686-7700 (2018). PubMed: 29931089
