Anti-DDX1抗体(ab70252)
Key features and details
- Rabbit polyclonal to DDX1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DDX1抗体
参阅全部 DDX1 一抗 -
描述
兔多克隆抗体to DDX1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human DDX1 (C terminal). Synthetic peptide corresponding to a region between residue 650 and the C-terminus (residue 697) of human DDX1. (AAH12739.1)
Database link: Q92499 -
阳性对照
- Whole cell lysate from Hela cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70252于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP | (1) |
Use at 3-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 3-5 µg/mg of lysate. |
靶标
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功能
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. -
组织特异性
Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. -
序列相似性
Belongs to the DEAD box helicase family. DDX1 subfamily.
Contains 1 B30.2/SPRY domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The helicase domain is involved in the stimulation of RELA transcriptional activity. -
翻译后修饰
Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR). -
细胞定位
Nucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. - Information by UniProt
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数据库链接
- Entrez Gene: 395249 Chicken
- Entrez Gene: 1653 Human
- Entrez Gene: 104721 Mouse
- Entrez Gene: 84474 Rat
- Omim: 601257 Human
- SwissProt: Q92499 Human
- SwissProt: Q91VR5 Mouse
- SwissProt: Q641Y8 Rat
see all -
别名
- ATP dependent helicase DDX1 antibody
- ATP-dependent RNA helicase DDX1 antibody
- DBP RB antibody
see all
图片
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Western blot - Anti-DDX1 antibody (ab70252)All lanes : Anti-DDX1 antibody (ab70252) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 83 kDa
Exposure time: 1 second -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (ab70252)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-DDX1 antibody (ab70252) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (ab70252)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70252 被引用在 2 文献中.
- Puri D et al. Comparative nuclear matrix proteome analysis of skeletal muscle cells in different cellular states. Cell Biol Int 45:580-598 (2021). PubMed: 33200434
- Wang IX et al. Human proteins that interact with RNA/DNA hybrids. Genome Res 28:1405-1414 (2018). PubMed: 30108179