Anti-DDT抗体(ab115785)
Key features and details
- Rabbit polyclonal to DDT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DDT抗体 -
描述
兔多克隆抗体to DDT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to a sequence at the C-terminal of Human DDT.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115785于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 13 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 13 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). -
序列相似性
Belongs to the MIF family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1652 Human
- Entrez Gene: 13202 Mouse
- Entrez Gene: 29318 Rat
- Omim: 602750 Human
- SwissProt: P30046 Human
- SwissProt: O35215 Mouse
- SwissProt: P80254 Rat
- Unigene: 656723 Human
see all -
别名
- D dopachrome decarboxylase antibody
- D dopachrome tautomerase antibody
- D-dopachrome decarboxylase antibody
see all
图片
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DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115785 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex DAB as the chromogen.
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All lanes : Anti-DDT antibody (ab115785) at 0.5 µg/ml
Lane 1 : rat liver tissues lysate
Lane 2 : rat testis tissues lysate
Lanes 3-4 : mouse liver tissues lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa -
All lanes : Anti-DDT antibody (ab115785) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat pancreas tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat lung tissue lysate
Predicted band size: 13 kDa -
ab115785 at 1µg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab115785 被引用在 1 文献中.
- Li X et al. Proteomic profiling identifies signatures associated with progression of precancerous gastric lesions and risk of early gastric cancer. EBioMedicine 74:103714 (2021). PubMed: 34818622